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  • Vulnerability in the Avalanche Capital: The Human Dimensions of Avalanche and Landslide Hazard in Juneau, Alaska  Provant, Zachary ( University of Oregon , 2024-08-07 ) In the United States, climate disasters kill thousands of people and cost billions of dollars each year. In 2023, the United States experienced 28 environmental disasters that cost more than one billion dollars each—the ...
  • Ecological Intensification of Oregon Hazelnut Orchards: Restoring Native Plant Communities in Shared Ecosystems  Lane-Massee, Marissa ( University of Oregon , 2024-08-07 ) The rapidly expanding Oregon hazelnut industry offers a unique opportunity for restoring ecosystem services to private lands that were historically oak-prairie dominated habitats. With typical orchard management consisting ...
  • Forward-looking approaches to rangeland restoration in a variable world  Batas, Lina ( University of Oregon , 2024-08-07 ) Ecological restoration is a powerful tool for repairing degraded ecosystems and promoting biodiversity and ecosystem functions. As global change drivers shift baseline conditions, forward-looking restoration approaches aim ...
  • AN ACCUMULATION OF CATASTROPHE: A POLITICAL ECONOMY OF WILDFIRE IN THE WESTERN UNITED STATES  Dockstader, Sue ( University of Oregon , 2024-03-25 ) This dissertation is an environmental sociological study of wildland fire in what is now the western United States. It examines wildfire management from roughly the 1900s to the present time employing a Marxist historical ...
  • Managing Life's Future: Species Essentialism and Evolutionary Normativity in Conservation Policy, Practice, and Imaginaries  Maggiulli, Katrina ( University of Oregon , 2024-01-10 ) Folk essentialist and normative understandings of species are not only prevalent in popular layperson communities, but also end up undergirding United States conservation policy and practice due to the simplistic clarity ...
  • Unsettled Ecologies: Alienated Species, Indigenous Restoration, and U.S. Empire in a Time of Climate Chaos  Fink, Lisa ( University of Oregon , 2024-01-10 ) This dissertation traces environmental thinking about invasive species from Western-colonial, diasporic settlers of color, and Indigenous perspectives within U.S. settler colonialism. Considering environmental discourses ...
  • Futuremaking in a Disaster Zone: Everyday Climate Change Adaptation amongst Quechua Women in the Peruvian Cordillera Blanca  Moulton, Holly ( University of Oregon , 2024-01-10 ) Indigenous women in Peru are often labeled “triply vulnerable” to climate change due to race, gender, and economic marginalization. Despite Peru’s focus on gender, Indigeneity, and intersectionality in national adaptation ...
  • Land Acts: Land's Agency in American Literature, Law, and History from the Colonial Period to Removal  Keeler, Kyle ( University of Oregon , 2024-01-10 ) This dissertation examines land’s agency and relationships to land in the places now known as the United States as these relationships appear in literature and law from early colonization to the removal period. Land Acts ...
  • PALEOTEMPERATURE, VEGETATION CHANGE, FIRE HISTORY, AND LAKE PRODUCTIVITY FOR THE LAST 14,500 YEARS AT GOLD LAKE, PACIFIC NORTHWEST, USA  Baig, Jamila ( University of Oregon , 2024-01-09 ) The postglacial history of vegetation, wildfire, and climate in the Cascade Range (Oregon) is only partly understood. This study uses high-resolution analysis from a 13-meter, 14,500-year sediment core from Gold Lake to ...
  • On Western Juniper Climate Relations  Reis, Schyler ( University of Oregon , 2022-10-26 ) Western juniper woodlands are highly sensitive to climate in terms of tree-ring growth, seedling establishment and range distribution. Understanding the dynamics of western juniper woodlands to changes in precipitation, ...
  • Stories We Tell, Stories We Eat: Mexican Foodways, Cultural Identity, and Ideological Struggle in Netflix’s Taco Chronicles  Sanchez, Bela ( University of Oregon , 2022-10-26 ) Food is a biological necessity imbued with numerous social, cultural, and economic implications for identity production and everyday meaning-making. Food television is a unique medium for the meanings of food and foodways ...
  • Soil Nutrient Additions Shift Orthopteran Herbivory and Invertebrate Community Composition  Altmire, Gabriella ( University of Oregon , 2022-10-26 ) Anthropogenic alterations to global pools of nitrogen and phosphorus are driving declines in plant diversity across grasslands. As such, concern over biodiversity loss has precipitated a host of studies investigating how ...
  • Multispecies Memoir: Self, Genre, and Species Justice in Contemporary Culture  Otjen, Nathaniel ( University of Oregon , 2022-10-04 ) Liberal humanism articulates an individual, rational, autonomous, universal, and singularly human subject that possesses various rights and freedoms. Although the imagined subject at the heart of liberal humanist philosophy ...
  • Understanding How Changes in Disturbance Regimes and Long-Term Climate Shape Ecosystem and Landscape Structure and Function  Wright, Jamie ( University of Oregon , 2022-10-04 ) Long-term and anthropic climatic change intersecting with disturbances alters ecosystem structure and function across spatiotemporal scales. Quantifying ecosystem responses can be convoluted, therefore utilizing multiproxy ...
  • Ikpíkyav (To Fix Again): Drawing From Karuk World Renewal To Contest Settler Discourses Of Vulnerability  Vinyeta, Kirsten ( University of Oregon , 2022-10-04 ) The Klamath River Basin of Northern California has historically been replete with fire-adapted ecosystems and Indigenous communities. For the Karuk Tribe, fire has been an indispensable tool for both spiritual practice and ...
  • Grassland Restoration in Heterogeneous, Changing, and Human Dominated Systems  Brambila, Alejandro ( University of Oregon , 2022-10-04 ) Ecological restoration is a powerful tool to promote biodiversity and ecosystem function. Understanding underlying system variability and directional change can help predict outcomes of restoration interventions. Spatial ...
  • Restoring What? And for Whom? Listening to Karuk Ecocultural Revitalization Practitioners and Uncovering Settler Logics in Ecological Restoration.  Worl, Sara ( University of Oregon , 2022-05-10 ) What does it mean to restore a landscape degraded by settler colonialism? How might a well intentionedprocess like ecological restoration end up causing harm from underlying settler colonial logics? This thesis explores ...
  • Instigating Communities of Solidarity: An Exploration of Participatory, Informal, Temporary Urbanisms  Meier, Briana ( University of Oregon , 2021-11-23 ) This dissertationexamines the potential for participatory, informal urbanisms to buildcollaborative relations across ontological, cultural, and political difference. This research contributes to thefield of urban, environmental ...
  • The Holy Oak School of Art and Ecology: A Proposal for Arts-Based Environmental Education Programming  Best, Krysta ( University of Oregon , 2021-11-23 ) The following is a proposal for arts-based environmental education programming in elementary schools, after-school programs, and day-camp programs, entitled the Holy School of Art and Ecology. Ecophenomenological, arts-based ...
  • Settler Colonial Listening and the Silence of Wilderness in the Boundary Waters Canoe Area  Hilgren, Bailey ( University of Oregon , 2021-11-23 ) The Boundary Waters Canoe Area soundscape in northern Minnesota has a long and contested history but is most often characterized today as a pristine and distinctly silent wilderness. This thesis traces the construction and ...

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Home > USC Columbia > Public Health, Arnold School of > SPH_ENVIRONMENTAL_HEALTH_SCIENCES > Environmental Health Sciences Theses and Dissertations

Environmental Health Sciences Theses and Dissertations

Theses/dissertations from 2023 2023.

Transdisciplinary Approaches to Community Engagement, Research Translation And Science Communication Through Application of the Socio-Environmental Report Card Process and Product in Murrells Inlet, South Carolina , Ekaterina Negodyaeva Altman

Producer Interviews and Analysis as a Tool for Science Communication and Research Implementation in Agriculture , Lacy Marie Barnette

Infection Intensity and Prevalence From Perkinsus Marinus In The Eastern Oyster, Crassostrea Virginica ( GMELIN 1791) and Their Association With Salinity, Temperature, and Sex In South Carolina Estuaries , Deja E. Best

Quantification Of The Internalization Of A Commercially Available Silver Nanoparticle To The Bacterium Escherichia Coli , Samantha McNeal Case

From Oceans to Public Health: Impact of Climate Change and Socioecological Factors on Non-cholera Vibrio Spp. , Karlen Enid Correa Vélez

Replenishment of Essential Natural Resources: An Assessment of the Ability of Freshwater Algae to Enhance Crop Quality and Soil Health , Lala Christina Ek

Integrated Wastewater Surveillance For Sars-cov-2 And Antibiotic Resistant Bacteria: Implications For Public Health And Environmental Management , Mirza Isanovic

Parent Perspectives on Barriers and Facilitators to Optimal Healthcare and Medical Screenings for Children With Down Syndrome in South Carolina , Vinita Oberoi Leedom

Customer Discovery and Applied Research and Development for an Environmental Nanotechnology , Paul Michael Vecchiarelli

Theses/Dissertations from 2022 2022

Comparative Assessment of the Fate and Toxicity Of Chemically and Biologically Synthesized Silver Nanoparticles Toward Juvenile Clams, Mercenaria Mercenaria , Amar Yasser Al-Rshim

Detection of Microcystin Aerosol Particles , Alexis D. Dabney

Economic Development, Political Processes and Climate Change Adaptation in the Solomon Islands , Adam Christopher Ereth

Describing the Impact of the Sales of Antibiotics on the Environment and in Population Health , Andrés Gaviria-Figueroa

Coastal Stormwater Pond Pollutants and the Potential for Development of Antimicrobial Resistance in Vibrio and Enterococcus Bacteria , Cassandra L. Horton

Xenobiotic Exposures on Women’s Reproductive Health , Maria Zubizarreta McClam

Monitoring Titanium Dioxide Engineered Particles in Rural and Urban Surface Waters , Md Mahmudun Nabi

The Impact of Framing on Natural Gas Pipeline Siting In Virginia , Ritvik Shukla

Female Reproductive Toxicity of Harmful Algal Blooms-Produced Toxin Microcystin , Yingzheng Wang

Theses/Dissertations from 2021 2021

Partitioning of Volatile Organic Compounds Into the Multiple Environmental Compartments , Jeonghyeon Ahn

Uptake, Acuumulation, and Toixicity of Silver Nanoparticles to Juvenile Bivalve Mollusc’s Hard Clam Mercenaria Mercenaria , Hanaa Radhi Jolan Al Hameed

A Spatial Risk Prediction Model for Drug Overdose , Parisa Bozorgi

The Interactive Effects of Nontoxic Levels of Microplastics - Textile Microfibers and Tire Wear Particles, on the Acute Toxicity of Bifenthrin in the Estuarine Grass Shrimp, Palaemonetes pugio , Xiaoxuan Fan

Assessing Stakeholder Needs and Preferences for Coastal Swimming Advisories in Charleston, South Carolina , Zachary Haynes Hart

Health Communication Blindspot: A Case Study of Harmful Algal Blooms in the South (HABITS) , Jaron Hoani King

Theses/Dissertations from 2020 2020

The Use of Polymer-Coated Magnetic Nanoparticles for Amelioration Oil Toxicity and Enhancement Microbial Remediation , Amjed Alabresm

Selenium, Platinum and Cerium Oxide Nanoparticles' Applications to Cancer and Fibrotic Diseases in Medicine , Badria Almurshidi

Temporal Differences in the Emission and Atmospheric Dispersion of Particulate Matter From Waste and Drinking Water Treatment Facilities , Abdulmalik Alsaif

Identifying Seasonal and Daily Variations in ARG-Containing Bioaerosols Generated During the Wastewater Treatment Process , Mirza Isanovic

Mechanistic Insight on Vibrio Gazogenes Mediated Intervention of Aflatoxin Biosynthesis in Aspergillus Flavus , Rubaiya Jesmin

Microbiome Targeted Therapies in Gulf War Illness , Diana Agnes Kimono

Bacterial Water Quality Monitoring as Citizen Science in Congaree National Park, South Carolina , Shea McCarthy

Understanding the Influence of Changing Climate on Mycotoxin Contamination of Food and Indoor Fungi-Mediated Respiratory Illness , Mayomi Helen Omebeyinje

Development, Validation, and Application of a Novel Method to Separate and Quantify Silver Intracellular Uptake in Pseudomonas aeruginosa , Mohammed A. Othman

Computational Tools for Analyzing Correlations Between Microbial Biological Diversity and Ecosystems , Behzad Torkian

Using a 3D In Vitro Ovarian Tissue Culture Model to Screen for the Female Reproductive Toxicity of Pharmaceutical Compounds , Jingshan Xu

Theses/Dissertations from 2019 2019

Microcystin Exposure and Liver Injury Outcomes in NAFLD , Muayad Albadrani

The Effects of Climate Change on the Ecotoxicology of Contaminants of Emerging Concern: Flame Retardants, Contemporary Use Pesticides and Pharmaceuticals Personal Care Products on the Estuarine Grass Shrimp, Palaemontes pugio , Rajaa Nouri Al-Yassein

Relative Importance of Ions and Particles in Silver Nanoparticle Uptake and Accumulation in Keystone Estuarine Organisms; Prorocentrum minimum and Crassostrea virginica , Shelby Victoria Butz

A Site Characterization and Assessment of the Bushy Park Reservoir, a Charleston-Area Drinking Water Source, and Its Surrounding Watersheds in Response to Community Concerns , Bradley Joseph Chandler

Nutrient Limitation of Phytoplankton in Lake Wateree, South Carolina: Implications for Future Water Quality Management , Kara M. Clyburn

Potassium Fertilization Effects on Yield for Wheat, Soybean, and Corn Grown in Coastal Plain Soils , Renada Davis

Revisiting the Standard Soil Test: The Potential of Macro- and Microbiological Measures to Improve Agricultural Health and Productivity , Gabriel Jacob Kenne

Investigating the Relationship of COPD, Lung Cancer, and Polycyclic Aromatic Hydrocarbons From Ambient Air Pollution , Daniel John Kilpatrick

Coming Together and Falling Apart: Producing and Contesting Place on the Atlanta Beltline , Jessica Martinez

The Health Implication and Application of Silver and Iron Oxide Nanoparticles , Sahar Pourhoseini

Study of the Inflammatory and Fibrotic Pathology in the Gut Following Microcystin Exposure Under Conditions of Non-Alcoholic Fatty Liver Disease. , Sutapa Sarkar

Role of Size, Concentration, and Natural Organic Matter on the Fate, Behavior, and Toxicity of Nanoparticle in Aquatic Environment , Mithun Sikder

Theses/Dissertations from 2018 2018

Effect Of Environmental Factors On The Aggregation Behavior Of Silver Nanoparticles In The Aquatic Environment , Kamelia Afshinnia

Environmental Links To Ectopic Kidney Diseases , Firas Alhasson

Understanding The Impact Of Land Use On Microbial Water Quality To Support Decisions For A Future Land Use Plan , Mohammed Tawfiq Aljassim

Linking Microbial Phylogenetic and Functional Gene Diversity to Microbial Mat Ecosystem Function Following Environmental Disturbance , Eva Christine Preisner

Inhibition Of Aflatoxin Biosynthesis With Vibrio Gazogenes , Phani Madhuri Gummadidala

Application Of Polymer-Coated Magnetic Nanoparticles For Oil Separation , Seyyedali Mirshahghassemi

Environmental Impacts on Enterococcus in Shem Creek, South Carolina, and Characterization of Changing Land Use , Maria Elizabeth Zubizarreta

Theses/Dissertations from 2017 2017

Mercury Bioaccumulation In Three Popular Subsistence And Recreational Estuarine Fishes From Southeastern U.S.A. , Taylor Cannon

A Study Of Hepatic Purinergic Receptor X7 Molecular Mechanisms In Ectopic Intestinal Inflammation In Environmental NAFLD , Varun Chandrashekaran

A Mechanistic Study of Micro RNA 21 Induced Hepatic Fibrosis and Use Of Sparstolonin B In Remediation Of Environmental NAFLD , Diptadip Dattaroy

Prenatal Methylmercury Exposure in a South Carolina Coastal Cohort , Alexis Donohue

Prenatal Methylmercury Exposure through Rice Consumption in Rural China , Chuan Hong

Endocrine Disruption in Largemouth Bass (Micropterus Salmoides) from a Pcb-Contaminated Reservoir , Stephanie B. LaPlaca

Exploratory Modeling of Environmental and Anthropogenic Impacts on Mercury in Fish Tissue for Select Watersheds in South Carolina , Julia McGuire

Population Dynamics of a Recently Described and Rare Plant Species: Stachys Caroliniana (Lamiaceae) , Shelby Moody

Theses/Dissertations from 2016 2016

Uptake of Nanoparticles by Vibrio Gazogenes , Shonda Renee Jones

Citrate Coated Silver Nanoparticles With Modulatory Effects On Aflatoxin Biosynthesis In Aspergillus Parasiticus , Chandrani Mitra

Theses/Dissertations from 2015 2015

Bacterial Communication and its Role as a Target for Nanoparticle-Based Antimicrobial Therapy , Kristen Publicover Miller

Comparing the Geography of Environmental Justice Guidelines, Fish Consumption Waterbodies, and Land Use/Land Cover in South Carolina , Matthew James Neet

High-Content Screening in Zebrafish Embryos Identifies Abamectin as a Potent Neurotoxicant , Tara Diane Raftery

Theses/Dissertations from 2014 2014

A Study of an Uncharacterized Penicillium Species Isolated from a HYPERSALINE Pond in San Salvador, Bahamas , Virginia Elizabeth Hopkins

Potential Stormwater Runoff Reductions by Incorporating Low Impact Development: Rocky Branch Watershed, Columbia, SC , Brett Sexton

Theses/Dissertations from 2013 2013

Estimation of Aerosolized Antibiotic Resistant Bacteria Concentrations from Wastewater Treatment Facilities Using Spatially-Based Dispersion Modeling , Renee Nichole Dickman

Fishing and Fish Consumption Patterns in the Gullah/Geechee Sea Island Population , Jamelle Heyward Ellis

Decision-Support Tool for Residential Pesticides in the South Carolina Coastal Zone , Lisa Claire Wickliffe

Adverse Outcome Pathways: A framework for Identifying Chemical Mode of Action During Zebrafish Embryogenesis , Krystle Yozzo

Theses/Dissertations from 2012 2012

G-Protein-Coupled Estrogen Receptor-1 (GPER): A Potential Target For Xenoestrogens During Vertebrate Embryogenesis. , Sumith Jayasinghe Balapanage

Data Management tools For Metagenomic Datasets, A Case Study: Characterization and Dysbiosis of Gut Microbiota In Apcmin/+ Mice With and Without Colon Tumors , Gargi Dayama

Influence of Insulin, Insulin-Like Growth Factor I and Sex Steroids On the Regulation of the Vitellogenin Receptor In Developing Oocytes of Largemouth Bass (Micropterus Salmoides) , Gustavo Dominguez

Developmental Toxicity of Organophosphate-Based Flame Retardants , Sean Patrick McGee

Identification of Quorum Sensing Genes in Halococcus Hamelinensis SP. Nov. 100Na1 , Eva Christine Preisner

Theses/Dissertations from 2011 2011

Identification of Quorum Sensing Genes in Hypersaline Microbial Mats , Benjamin Senyo Bey

Examining Fluorescent Decay Rates For Microbial Source Tracking In South Carolina Blackwater Systems , Francine Rosemarie Davis

Determination of Urban Land Use Variables That Accurately Predict Fecal Coliform Levels In Shellfish Harvesting Waters Within Estuarine Salt Marsh Ecosystems of the Southeastern United States of America. , Darrell Dewayne Dodgens

Microbial Antibiotic Resistance and Integron Gene Distribution Within An Urbanized Coastal Estuarine Ecosystem , Miguel Ignacio Uyaguari

Theses/Dissertations from 2010 2010

An In Vitro Assessment of The Potential for Engineered Nanoparticles to Modulate Innate Immunity , Leslyn Brusch-Richardson

Growth and Developmental Enhancement of Poplar ( Populus Deltoides X Nigra Op-367) by Associated Endophytic Bacteria , Adam Spencer Hoffman

American Alligator Fecal Coliform and Pathogen Identification, Genotypic Fingerprinting, and REP-PCR Bacterial Source Tracking in South Carolina , Michelle Anne Johnston

Sex Specific Gene Expression Profiles In Fibrosis and Copd , Sean Patrick McGee

Lake Wateree - Getting Out of the Lake and Into the Watershed: A Study of Volunteer Monitoring Efforts, Water Quality, and Community Outreach , Sara Powell

A Comparison of Vegtation Indices and Conventional Ecotoxicological Plant Growth Metrics to Assess the Lethal and Sublethal Effects of Atrazine on Smooth Cordgrass,'Spartina alterniflora' , Cecile Hart Scott

Investigation of Low Impact Development Practices For Coastal South Carolina: Oak Terrace Preserve , Ann Elisabeth Vandiver

Theses/Dissertations from 2009 2009

Temporal Expression and Transcriptional Regulation of the Vitellogenin Receptor Gene In Largemouth Bass (Micropterus Salmoides) , Gustavo Dominguez

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Theses and Dissertations (Environmental Sciences)

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  • An assessment of the efficiency of adsorbents produced from a mix of lemon and orange skins in the removal of metals from wastewater  Mbaya, Tresor Kabongo ( 2023-10-01 ) Waste materials generated from various agricultural products, processes, and activities have been widely investigated as potential biomass to produce adsorbents with the capacity to remove heavy metals from effluents of ...
  • Investigating the impact of ISO 14001 on waste minimisation within South Africa  Rabalao, Kokesto Johannes ( 2023-12-19 ) Waste and pollution emanating from industrial activities is a primary global concern, prompting industries to seek solutions to reduce environmental impacts. Among other proposed environmental measures to reduce environmental ...
  • Integration of education for sustainable development into the life sciences curriculum : teachers’ pedagogical content knowledge and professional development  Munasi, Khathutshelo Ronald ( 2024-01 ) This study explores enhancing Life Science teachers' pedagogical content knowledge (PCK) through professional development to facilitate seamless integration of Education for Sustainable Development (EE/ESD) into the ...
  • Solid waste management through environmental education initiatives : a case of Amathole East District  Nyathi, Isiah ( 2023-12 ) Disposing of solid waste is one of the most serious and controversial issues facing countries around the world. The problem is caused by daily human activities. The main aim of this qualitative study was to explore solid ...
  • Assessing Signals of Mesopredator Release under Two Apex Predator Regimes in Tswalu Kalahari Reserve, South Africa  Collins, Roxanne Natasha ( 2023-10-31 ) Predators have a considerable influence on the structure and functioning of the ecosystems in which they occur, however their numbers have declined, and continue to do so. The eradication of top-level predators has been ...
  • Reconnoitering sand mining impacts in the Eastern Cape Province of South Africa  Mngeni, Asangonga ( 2021-11 ) dissertation presents an account of the impacts of sand mining activities in the province of the Eastern Cape of South Africa. The theoretical framework and methodology of the dissertation are based on the dialectical ...
  • Biological evaluation of ethnobotanical selected medicinal plants used in the management of male sexual health  Nemandalali, Tsumbedzo ( 2023-11 ) Backround: Ethnobatanical knowledge is mostly confined among the locals, hence proper enquiry, and documentation of medicinal plants in a specific area can aid the constantly evolving scientific community in protecting ...
  • Functional genomic and metabolomics characterisation of bioleaching fungi from mine tailings  Nkuna, Rosina ( 2023-12 ) Bioleaching is a biological and an environmentally friendly alternative to traditional leaching methods for extracting metals from ores or wastes. However, its reliance on microorganism, which are sensitive to bioleaching ...
  • The ecological assessment of the influence of anthropogenic activities on Palala River, Limpopo, South Africa  Sibiya, Sifundile ( 2023-08 ) Water quality integrity deterioration is a severe global issue due to urbanisation, population growth, pollution and other anthropogenic activities threatening freshwater integrity. Anthropogenic activities alter freshwater ...
  • The impact of licensing on selected hazardous waste disposal facilities in South Africa  Dlamini, Zinhle Teresa (Mbili) ( 2023-02 ) This research evaluates the impact of waste licensing conditions on the operations of three selected hazardous waste disposal facilities, particularly in terms of compliance with conditions of the issued waste management ...
  • Factors affecting sustainable water use in South Africa : a case of Cape Town  Musariri, Yeukai ( 2023-01-31 ) Cape Town's water shortages continue unabated, with most residents resorting to unsafe water sources. The continuation of the water shortages has reached crisis level, which signals challenges in the provision of clean, ...
  • Sustainability of indigenous methods of solid waste management in Sikhunyani Village, Limpopo Province, South Africa  Rabonda, Lethabo Caroline ( 2023-02-24 ) Most rural areas do not have access to basic amenities such as municipal solid waste (MSW) disposal services and use various indigenous methods of solid waste management and disposal. Most communities in developing countries ...
  • Inclusion of local community farmers in the last-mile supply chain processes of luxury wildlife tourism destinations  Van den Berg, Hugo ( 2022-12-01 ) The fresh produce supply chains' content, nature, and characteristics for luxury tourism wildlife destinations in South Africa and sub-Saharan Africa are unknown. There is no synthesised document that compares the current ...
  • A case study for wetland restoration towards socio-ecological appreciation of natural resources in Kwazulu-Natal (South Africa)  Acker, Cherise ( 2022-11 ) Social cognitive theory suggests that in order to bring about change in society an environment conducive to change is imperative. This theory was investigated within a socio-ecological framework using a comparative study ...
  • Assessing the abundance of Cryptosporidium and Giardia spp. in borehole water close to eMbalenhle wastewater treatment plant and associated human health impacts  Makhombothi, Busisiwe Fezile ( 2023-03 ) Groundwater quality is often an overlooked aspect and due to this oversight, groundwater remains vulnerable to contamination by such sources as wastewater treatment plants (WWTPs). Even though most WWTPs in South Africa ...
  • An assessment of the occurrence and removal options of microplastics in wastewater treatment processes at the City Ekurhuleni and Midvaal in South Africa  Mphaga, Tendani ( 2023-03-03 ) The main aim of the study was to investigate the occurrence and removal of microplastics in wastewater treatment processes in Ekurhuleni and Midvaal in South Africa. The amount of microplastics and chemical composition ...
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Dissertation_Sachs_Maria.pdf - Accepted Version
Bereitstellung unter der CC-Lizenz: .

Protists, unicellular eukaryotes, are one of the most diverse (if not the most diverse) group of organisms on the planet and are widely distributed over the eukaryotic tree of live. They exhibit a ubiquitous distribution upon many terrestrial and aquatic ecosystems. Additionally, their ecological roles span across many different trophic modes, from phototrophic primary producers like phytoplankton, to parasites, symbionts and predators, significantly involved into ecological nutrient cycling. In recent years, molecular techniques have unveiled an impressive array of previously unknown protists, changing our perception of their ecological roles and diversity in global ecosystems. The primary aim of the present study was to explore the diversity with regard to taxonomic and functional composition. By combining amplicon sequencing with modern taxonomic methods such as cultivation-based approaches and microscopy, our objective was to expand knowledge of protist diversity in brackish and freshwater habitats. This included exploring the protist communities in their entirety as well as investigating two specific communities closely associated with amphibian species. We selected two regions in the southern Baltic Sea differing in several abiotic factors (Fehmarnbelt and Oderbank), as a model for studying benthic protist communities in brackish-water environments. The study offered novel insights into brackish-water communities, that have not been investigated in this magnitude and might hint at potential correlations between specific protist groups and environmental parameters. This study also has an applied aspect as it was intended to serve as a baseline study to monitor the effect of ground fishing in future. Using a very strictly filtered amplicon sequencing dataset, we found no overlap in taxonomic composition between the two selected regions. While ciliates exhibited remarkable diversity in both regions, marine alveolates belonging to the MALV group displayed the highest read abundances. Previous studies have shown that this group of organisms, with an assumed parasitic life style, is highly abundant in marine environmental datasets, but have not yet been recorded from brackishwater habitats. However, as they are mostly uncultured, not much is known about their ecology. In freshwater environments but also in amphibian hosts, protist parasites and commensals are known to be common. As an example, we wanted to get more insight into the trophic mode of parasitism by investigating the presence of Batrachochytrium dendrobatidis (Bd) and other amphibian parasites in the green toad (Bufotes viridis) and syntopic amphibians. Therefore, we combined DNA metabarcoding with a qPCR survey. The results revealed the presence of Bd in green toad populations, highlighting the potential threat of this deadly pathogen to amphibian populations in the investigated region. Additionally, the study identified other parasitic organisms, mainly protists, inhabiting the gut of the amphibians. As an antagonist to the detrimental effects of the presence of protists in connection with amphibians, we were interested in the occurrence of the unicellular alga Oophila in German breeding waters of Rana dalmatina. The alga is known for its close association with various amphibians, forming a kind of mutualistic relationship. Our study offers valuable insights into the diversity of micro-eukaryotes, with a particular focus on green algae, inhabiting amphibian clutches. Through DNA metabarcoding, we uncovered a wide array of protist taxa colonizing these amphibian egg masses, highlighting the ecological complexity of this specific habitat. Importantly, the study revealed substrate-specific preferences among these protists - clutches are inhabited by a unique protist community. Additionally, we aimed to disentangle parts of the uncertain taxonomy of Oophila, showing that a certain clade is exceptionally frequent in amphibian clutches, also in our investigated specimens. These results emphasize how taxonomic studies play a crucial role in enhancing the accuracy and reliability of public databases used in environmental sequencing studies. Modern taxonomy provides essential reference points for researchers to correctly classify and interpret the vast diversity of genetic sequences encountered in various ecosystems. To highlight this aspect further more, we investigated two taxonomic groups in more detail, to revise and/or expand their taxonomy. The genus Goniomonas (sensu lato) is not only the single taxon of cryptophytes without a plastid, it also shows a deep genetic divergence between the freshwater and marine strains. Even though it is a very frequently observed heterotrophic flagellate, only few species have been described yet, mainly on the basis of morphologic observations. The objective of our study was to revise the genus Goniomonas and offer a suitable Neotype. In the course of the study it became evident that the genus had be be split into several new genera. We based the introduction of Limnogoniomonas gen. nov., Goniomonas and Aquagoniomonas gen. nov., Neptunogoniomonas gen. nov., Marigoniomonas gen. nov, Thalassogoniomonas gen. nov., Poseidogoniomonas gen. nov. and Cosmogoniomonas gen. nov. on both morphological and phylogenetic investigations. The taxonomy of the bicosoecid Cafeteria has been recently revised. With our study we aimed to resolve the species diversity of the genus furthermore, additionally by adding autecological experiments into an integrative taxonomic approach. In this way, we were able to describe several new species of Cafeteria including one from the Baltic Sea. As those sequences are or will deposted in public databases, this thesis shows the close connection between modern taxonomy and environmental sequencing.

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Item Type: Thesis (PhD thesis)
Translated title:
TitleLanguage
Ökologie und Diversität von Protisten in Sedimenten und amphibienassoziierten LebensräumenUNSPECIFIED
AbstractLanguage
Protisten, einzellige Eukaryoten, sind eine der vielfältigsten (wenn nicht die vielfältigste) Gruppe von Organismen auf diesem Planeten und sind weit über den eukaryotischen Stammbaum des Lebens verteilt. Sie weisen eine ubiquitäre Verbreitung in vielen terrestrischen und aquatischen Ökosystemen auf. Darüber hinaus umfassen ihre ökologischen Rollen viele verschiedene trophische Modi, von phototrophen Primärproduzenten wie Phytoplankton bis hin zu Parasiten, Symbionten und Prädatoren, die wesentlich in den ökologischen Nährstoffkreislauf eingebunden sind. In den letzten Jahren haben molekulare Techniken eine beeindruckende Anzahl zuvor unbekannter Protistenarten aufgedeckt, was unsere Wahrnehmung ihrer ökologischen Rollen und Vielfalt in globalen Ökosystemen verändert hat. Das Hauptziel der vorliegenden Studie war es, die Diversität hinsichtlich der taxonomischen und funktionellen Zusammensetzung zu erforschen. Durch die Kombination von Amplicon-Sequenzierung mit modernen taxonomischen Methoden wie kulturbasierten Ansätzen und Mikroskopie war unser Ziel, das Wissen über die Protistendiversität in Brack- und Süßwasserlebensräumen zu erweitern. Dies umfasste die Untersuchung der Protistengemeinschaften in ihrer Gesamtheit sowie die Erforschung zweier spezifischer Gemeinschaften, die eng mit Amphibienarten assoziiert sind. Wir wählten zwei Regionen in der südlichen Ostsee, die sich in mehreren abiotischen Faktoren unterscheiden (Fehmarnbelt und Oderbank), als Modell zur Untersuchung benthischer Protistengemeinschaften in Brackwassersystemen. Die Studie bot neuartige Einblicke in Brackwassergemeinschaften, die in diesem Ausmaß noch nicht untersucht wurden, und könnte auf potenzielle Korrelationen zwischen spezifischen Protistengruppen und Umweltparametern hinweisen. Diese Studie hat auch einen angewandten Aspekt, da sie als Grundlagen-Studie zur Überwachung der Auswirkungen der grundberührenden Fischerei auf benthische Biozoenosen dienen soll. Mithilfe eines sehr streng gefilterten Amplicon-Sequenzierungsdatensatzes fanden wir keine Überlappung in der taxonomischen Zusammensetzung zwischen den beiden ausgewählten Regionen. Während Ciliaten in beiden Regionen eine bemerkenswerte Diversität zeigten, wiesen marine Alveolaten der MALV-Gruppe die höchste Anzahl an Reads im Datensatz auf. Frühere Studien haben bereits gezeigt, dass diese Gruppe von Organismen, von denen angenommen wird, dass sie einen parasitischen Lebensstil haben, in marinen Umweltdatensätzen hochabundant ist, bisher ist dieses aber noch nicht in Brackwasserlebensräumen dokumentiert wurde. Da sie größtenteils nicht kultiviert sind, ist zur Zeit noch wenig über ihre Ökologie bekannt. In Süßwasserumgebungen, aber auch in Amphibienwirten, sind Protistenparasiten und -Kommensale häufig. Als Beispiel wollten wir mehr Einblick in den trophischen Modus des Parasitismus gewinnen, indem wir das Vorhandensein von Batrachochytrium dendrobatidis (Bd) und anderen Amphibienparasiten in der Wechselkröte (Bufotes viridis) und syntopischen Amphibien untersucht haben. Daher kombinierten wir DNA-Metabarcoding mit einer qPCR Analyse. Die Ergebnisse zeigten das Vorhandensein von Bd in Wechselkrötenpopulationen und hoben die potenzielle Bedrohung dieses tödlichen Erregers für Amphibienpopulationen in der untersuchten Region hervor. Zusätzlich identifizierte die Studie andere parasitäre Organismen, hauptsächlich Protisten, die den Darm der Amphibien bewohnen. Als Antagonist zu den schädlichen Auswirkungen von Protisten in Verbindung mit Amphibien waren wir an der Vorkommen der einzelligen Alge Oophila in deutschen Laichgewässern des Springfroschs (Rana dalmatina) interessiert. Die Alge ist für ihre enge Assoziation mit verschiedenen Amphibien bekannt und bildet eine Art mutualistische Beziehung mit diesen. Unsere Studie bietet wertvolle Einblicke in die Diversität der Mikro-Eukaryoten, mit einem besonderen Fokus auf Grünalgen, die Amphibienlaich bewohnen. Durch DNA-Metabarcoding deckten wir eine breite Palette von Protistentaxa auf, die Amphibienlaich besiedeln, und hoben die ökologische Komplexität dieses spezifischen Lebensraums hervor. Die Studie offenbarte substratspezifische Präferenzen bei diesen Protisten - Laichballen werden von einer einzigartigen Protistengemeinschaft bewohnt. Darüber hinaus zielten wir darauf ab, Teile der unsicheren Taxonomie von Oophila zu entwirren, und zeigten, dass eine bestimmte Klade außergewöhnlich häufig in Amphibienlaich vorkommt. Diese Ergebnisse betonen, wie taxonomische Studien eine entscheidende Rolle bei der Verbesserung der Genauigkeit und Zuverlässigkeit öffentlicher Datenbanken spielen, die in Umweltsequenzierungsstudien verwendet werden. Moderne Taxonomie bietet wesentliche Referenzpunkte für Forscher, um die enorme Vielfalt an genetischen Sequenzen, die in verschiedenen Ökosystemen gefunden werden, korrekt zu klassifizieren und zu interpretieren. Um diesen Aspekt weiter zu betonen, untersuchten wir zwei taxonomische Gruppen im Detail, um ihre Taxonomie zu überarbeiten und/oder zu erweitern. Die Gattung Goniomonas (sensu lato) ist nicht nur das einzige Taxon der Cryptophyten ohne Plastid, sie zeigt auch eine tiefe genetische Divergenz zwischen den Süßwasser- und Meeresstämmen. Obwohl es sich um eine sehr häufig beobachtete heterotrophe Flagellatengruppe handelt, wurden bisher nur wenige Arten beschrieben, hauptsächlich auf der Grundlage morphologischer Beobachtungen. Ziel unserer Studie war es, die Gattung Goniomonas zu überarbeiten und einen geeigneten Neotypus vorzuschlagen. Im Verlauf der Studie wurde deutlich, dass die Gattung in mehrere neue Gattungen aufgesplittet werden musste. Wir basierten die Einführung von Limnogoniomonas gen. nov., Goniomonas und Aquagoniomonas gen. nov., Neptunogoniomonas gen. nov., Marigoniomonas gen. nov., Thalassogoniomonas gen. nov., Poseidogoniomonas gen. nov. und Cosmogoniomonas gen. nov. sowohl auf morphologischen als auch phylogenetischen Untersuchungen. Die Taxonomie der Bicosoeciden Gattung Cafeteria wurde kürzlich revidiert. Mit unserer Studie zielten wir darauf ab, die Artenvielfalt der Gattung weiter aufzuschlüsseln - zusätzlich durch die Hinzufügen autökologischer Experimente in einem integrativen taxonomischen Ansatz. Auf diese Weise konnten wir mehrere neue Arten von Cafeteria beschreiben, einschließlich einer aus der Ostsee. Da diese Sequenzen in öffentlichen Datenbanken hinterlegt sind oder hinterlegt werden, zeigt diese Arbeit die enge Verbindung zwischen moderner Taxonomie und Umweltsequenzierung.German
CreatorsEmailORCIDORCID Put Code
Sachs, Maria Sophie[email protected] UNSPECIFIED
ContributionNameEmail
Thesis advisorArndt, Hartmut[email protected]
KeywordsLanguage
ProtistologyEnglish
EcologyEnglish
NameAcademic Title
Arndt, HartmutProf. Dr.

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