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• How to Write a Discussion Section | Tips & Examples

How to Write a Discussion Section | Tips & Examples

Published on August 21, 2022 by Shona McCombes . Revised on July 18, 2023.

The discussion section is where you delve into the meaning, importance, and relevance of your results .

It should focus on explaining and evaluating what you found, showing how it relates to your literature review and paper or dissertation topic , and making an argument in support of your overall conclusion. It should not be a second results section.

There are different ways to write this section, but you can focus your writing around these key elements:

• Summary : A brief recap of your key results
• Interpretations: What do your results mean?
• Implications: Why do your results matter?
• Limitations: What can’t your results tell us?
• Recommendations: Avenues for further studies or analyses

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Table of contents

What not to include in your discussion section, step 1: summarize your key findings, step 2: give your interpretations, step 3: discuss the implications, step 4: acknowledge the limitations, step 5: share your recommendations, discussion section example, other interesting articles, frequently asked questions about discussion sections.

There are a few common mistakes to avoid when writing the discussion section of your paper.

• Don’t introduce new results: You should only discuss the data that you have already reported in your results section .
• Don’t make inflated claims: Avoid overinterpretation and speculation that isn’t directly supported by your data.
• Don’t undermine your research: The discussion of limitations should aim to strengthen your credibility, not emphasize weaknesses or failures.

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Start this section by reiterating your research problem and concisely summarizing your major findings. To speed up the process you can use a summarizer to quickly get an overview of all important findings. Don’t just repeat all the data you have already reported—aim for a clear statement of the overall result that directly answers your main research question . This should be no more than one paragraph.

Many students struggle with the differences between a discussion section and a results section . The crux of the matter is that your results sections should present your results, and your discussion section should subjectively evaluate them. Try not to blend elements of these two sections, in order to keep your paper sharp.

• The results indicate that…
• The study demonstrates a correlation between…
• This analysis supports the theory that…
• The data suggest that…

The meaning of your results may seem obvious to you, but it’s important to spell out their significance for your reader, showing exactly how they answer your research question.

The form of your interpretations will depend on the type of research, but some typical approaches to interpreting the data include:

• Identifying correlations , patterns, and relationships among the data
• Discussing whether the results met your expectations or supported your hypotheses
• Contextualizing your findings within previous research and theory
• Explaining unexpected results and evaluating their significance
• Considering possible alternative explanations and making an argument for your position

You can organize your discussion around key themes, hypotheses, or research questions, following the same structure as your results section. Alternatively, you can also begin by highlighting the most significant or unexpected results.

• In line with the hypothesis…
• Contrary to the hypothesized association…
• The results contradict the claims of Smith (2022) that…
• The results might suggest that x . However, based on the findings of similar studies, a more plausible explanation is y .

As well as giving your own interpretations, make sure to relate your results back to the scholarly work that you surveyed in the literature review . The discussion should show how your findings fit with existing knowledge, what new insights they contribute, and what consequences they have for theory or practice.

Ask yourself these questions:

• Do your results support or challenge existing theories? If they support existing theories, what new information do they contribute? If they challenge existing theories, why do you think that is?
• Are there any practical implications?

Your overall aim is to show the reader exactly what your research has contributed, and why they should care.

• These results build on existing evidence of…
• The results do not fit with the theory that…
• The experiment provides a new insight into the relationship between…
• These results should be taken into account when considering how to…
• The data contribute a clearer understanding of…
• While previous research has focused on  x , these results demonstrate that y .

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Even the best research has its limitations. Acknowledging these is important to demonstrate your credibility. Limitations aren’t about listing your errors, but about providing an accurate picture of what can and cannot be concluded from your study.

Limitations might be due to your overall research design, specific methodological choices , or unanticipated obstacles that emerged during your research process.

Here are a few common possibilities:

• If your sample size was small or limited to a specific group of people, explain how generalizability is limited.
• If you encountered problems when gathering or analyzing data, explain how these influenced the results.
• If there are potential confounding variables that you were unable to control, acknowledge the effect these may have had.

After noting the limitations, you can reiterate why the results are nonetheless valid for the purpose of answering your research question.

• The generalizability of the results is limited by…
• The reliability of these data is impacted by…
• Due to the lack of data on x , the results cannot confirm…
• The methodological choices were constrained by…
• It is beyond the scope of this study to…

Based on the discussion of your results, you can make recommendations for practical implementation or further research. Sometimes, the recommendations are saved for the conclusion .

Suggestions for further research can lead directly from the limitations. Don’t just state that more studies should be done—give concrete ideas for how future work can build on areas that your own research was unable to address.

• Further research is needed to establish…
• Future studies should take into account…
• Avenues for future research include…

If you want to know more about AI for academic writing, AI tools, or research bias, make sure to check out some of our other articles with explanations and examples or go directly to our tools!

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In the discussion , you explore the meaning and relevance of your research results , explaining how they fit with existing research and theory. Discuss:

• Your  interpretations : what do the results tell us?
• The  implications : why do the results matter?
• The  limitation s : what can’t the results tell us?

The results chapter or section simply and objectively reports what you found, without speculating on why you found these results. The discussion interprets the meaning of the results, puts them in context, and explains why they matter.

In qualitative research , results and discussion are sometimes combined. But in quantitative research , it’s considered important to separate the objective results from your interpretation of them.

In a thesis or dissertation, the discussion is an in-depth exploration of the results, going into detail about the meaning of your findings and citing relevant sources to put them in context.

The conclusion is more shorter and more general: it concisely answers your main research question and makes recommendations based on your overall findings.

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The purpose of the discussion section is to interpret and describe the significance of your findings in relation to what was already known about the research problem being investigated and to explain any new understanding or insights that emerged as a result of your research. The discussion will always connect to the introduction by way of the research questions or hypotheses you posed and the literature you reviewed, but the discussion does not simply repeat or rearrange the first parts of your paper; the discussion clearly explains how your study advanced the reader's understanding of the research problem from where you left them at the end of your review of prior research.

Annesley, Thomas M. “The Discussion Section: Your Closing Argument.” Clinical Chemistry 56 (November 2010): 1671-1674; Peacock, Matthew. “Communicative Moves in the Discussion Section of Research Articles.” System 30 (December 2002): 479-497.

Importance of a Good Discussion

The discussion section is often considered the most important part of your research paper because it:

• Most effectively demonstrates your ability as a researcher to think critically about an issue, to develop creative solutions to problems based upon a logical synthesis of the findings, and to formulate a deeper, more profound understanding of the research problem under investigation;
• Presents the underlying meaning of your research, notes possible implications in other areas of study, and explores possible improvements that can be made in order to further develop the concerns of your research;
• Highlights the importance of your study and how it can contribute to understanding the research problem within the field of study;
• Presents how the findings from your study revealed and helped fill gaps in the literature that had not been previously exposed or adequately described; and,
• Engages the reader in thinking critically about issues based on an evidence-based interpretation of findings; it is not governed strictly by objective reporting of information.

Annesley Thomas M. “The Discussion Section: Your Closing Argument.” Clinical Chemistry 56 (November 2010): 1671-1674; Bitchener, John and Helen Basturkmen. “Perceptions of the Difficulties of Postgraduate L2 Thesis Students Writing the Discussion Section.” Journal of English for Academic Purposes 5 (January 2006): 4-18; Kretchmer, Paul. Fourteen Steps to Writing an Effective Discussion Section. San Francisco Edit, 2003-2008.

Structure and Writing Style

I.  General Rules

These are the general rules you should adopt when composing your discussion of the results :

• Do not be verbose or repetitive; be concise and make your points clearly
• Avoid the use of jargon or undefined technical language
• Follow a logical stream of thought; in general, interpret and discuss the significance of your findings in the same sequence you described them in your results section [a notable exception is to begin by highlighting an unexpected result or a finding that can grab the reader's attention]
• Use the present verb tense, especially for established facts; however, refer to specific works or prior studies in the past tense
• If needed, use subheadings to help organize your discussion or to categorize your interpretations into themes

II.  The Content

The content of the discussion section of your paper most often includes :

• Explanation of results : Comment on whether or not the results were expected for each set of findings; go into greater depth to explain findings that were unexpected or especially profound. If appropriate, note any unusual or unanticipated patterns or trends that emerged from your results and explain their meaning in relation to the research problem.
• References to previous research : Either compare your results with the findings from other studies or use the studies to support a claim. This can include re-visiting key sources already cited in your literature review section, or, save them to cite later in the discussion section if they are more important to compare with your results instead of being a part of the general literature review of prior research used to provide context and background information. Note that you can make this decision to highlight specific studies after you have begun writing the discussion section.
• Deduction : A claim for how the results can be applied more generally. For example, describing lessons learned, proposing recommendations that can help improve a situation, or highlighting best practices.
• Hypothesis : A more general claim or possible conclusion arising from the results [which may be proved or disproved in subsequent research]. This can be framed as new research questions that emerged as a consequence of your analysis.

III.  Organization and Structure

Keep the following sequential points in mind as you organize and write the discussion section of your paper:

• Think of your discussion as an inverted pyramid. Organize the discussion from the general to the specific, linking your findings to the literature, then to theory, then to practice [if appropriate].
• Use the same key terms, narrative style, and verb tense [present] that you used when describing the research problem in your introduction.
• Begin by briefly re-stating the research problem you were investigating and answer all of the research questions underpinning the problem that you posed in the introduction.
• Describe the patterns, principles, and relationships shown by each major findings and place them in proper perspective. The sequence of this information is important; first state the answer, then the relevant results, then cite the work of others. If appropriate, refer the reader to a figure or table to help enhance the interpretation of the data [either within the text or as an appendix].
• Regardless of where it's mentioned, a good discussion section includes analysis of any unexpected findings. This part of the discussion should begin with a description of the unanticipated finding, followed by a brief interpretation as to why you believe it appeared and, if necessary, its possible significance in relation to the overall study. If more than one unexpected finding emerged during the study, describe each of them in the order they appeared as you gathered or analyzed the data. As noted, the exception to discussing findings in the same order you described them in the results section would be to begin by highlighting the implications of a particularly unexpected or significant finding that emerged from the study, followed by a discussion of the remaining findings.
• Before concluding the discussion, identify potential limitations and weaknesses if you do not plan to do so in the conclusion of the paper. Comment on their relative importance in relation to your overall interpretation of the results and, if necessary, note how they may affect the validity of your findings. Avoid using an apologetic tone; however, be honest and self-critical [e.g., in retrospect, had you included a particular question in a survey instrument, additional data could have been revealed].
• The discussion section should end with a concise summary of the principal implications of the findings regardless of their significance. Give a brief explanation about why you believe the findings and conclusions of your study are important and how they support broader knowledge or understanding of the research problem. This can be followed by any recommendations for further research. However, do not offer recommendations which could have been easily addressed within the study. This would demonstrate to the reader that you have inadequately examined and interpreted the data.

IV.  Overall Objectives

The objectives of your discussion section should include the following: I.  Reiterate the Research Problem/State the Major Findings

Briefly reiterate the research problem or problems you are investigating and the methods you used to investigate them, then move quickly to describe the major findings of the study. You should write a direct, declarative, and succinct proclamation of the study results, usually in one paragraph.

II.  Explain the Meaning of the Findings and Why They are Important

No one has thought as long and hard about your study as you have. Systematically explain the underlying meaning of your findings and state why you believe they are significant. After reading the discussion section, you want the reader to think critically about the results and why they are important. You don’t want to force the reader to go through the paper multiple times to figure out what it all means. If applicable, begin this part of the section by repeating what you consider to be your most significant or unanticipated finding first, then systematically review each finding. Otherwise, follow the general order you reported the findings presented in the results section.

III.  Relate the Findings to Similar Studies

No study in the social sciences is so novel or possesses such a restricted focus that it has absolutely no relation to previously published research. The discussion section should relate your results to those found in other studies, particularly if questions raised from prior studies served as the motivation for your research. This is important because comparing and contrasting the findings of other studies helps to support the overall importance of your results and it highlights how and in what ways your study differs from other research about the topic. Note that any significant or unanticipated finding is often because there was no prior research to indicate the finding could occur. If there is prior research to indicate this, you need to explain why it was significant or unanticipated. IV.  Consider Alternative Explanations of the Findings

It is important to remember that the purpose of research in the social sciences is to discover and not to prove . When writing the discussion section, you should carefully consider all possible explanations for the study results, rather than just those that fit your hypothesis or prior assumptions and biases. This is especially important when describing the discovery of significant or unanticipated findings.

V.  Acknowledge the Study’s Limitations

It is far better for you to identify and acknowledge your study’s limitations than to have them pointed out by your professor! Note any unanswered questions or issues your study could not address and describe the generalizability of your results to other situations. If a limitation is applicable to the method chosen to gather information, then describe in detail the problems you encountered and why. VI.  Make Suggestions for Further Research

You may choose to conclude the discussion section by making suggestions for further research [as opposed to offering suggestions in the conclusion of your paper]. Although your study can offer important insights about the research problem, this is where you can address other questions related to the problem that remain unanswered or highlight hidden issues that were revealed as a result of conducting your research. You should frame your suggestions by linking the need for further research to the limitations of your study [e.g., in future studies, the survey instrument should include more questions that ask..."] or linking to critical issues revealed from the data that were not considered initially in your research.

NOTE: Besides the literature review section, the preponderance of references to sources is usually found in the discussion section . A few historical references may be helpful for perspective, but most of the references should be relatively recent and included to aid in the interpretation of your results, to support the significance of a finding, and/or to place a finding within a particular context. If a study that you cited does not support your findings, don't ignore it--clearly explain why your research findings differ from theirs.

V.  Problems to Avoid

• Do not waste time restating your results . Should you need to remind the reader of a finding to be discussed, use "bridge sentences" that relate the result to the interpretation. An example would be: “In the case of determining available housing to single women with children in rural areas of Texas, the findings suggest that access to good schools is important...," then move on to further explaining this finding and its implications.
• As noted, recommendations for further research can be included in either the discussion or conclusion of your paper, but do not repeat your recommendations in the both sections. Think about the overall narrative flow of your paper to determine where best to locate this information. However, if your findings raise a lot of new questions or issues, consider including suggestions for further research in the discussion section.
• Do not introduce new results in the discussion section. Be wary of mistaking the reiteration of a specific finding for an interpretation because it may confuse the reader. The description of findings [results section] and the interpretation of their significance [discussion section] should be distinct parts of your paper. If you choose to combine the results section and the discussion section into a single narrative, you must be clear in how you report the information discovered and your own interpretation of each finding. This approach is not recommended if you lack experience writing college-level research papers.
• Use of the first person pronoun is generally acceptable. Using first person singular pronouns can help emphasize a point or illustrate a contrasting finding. However, keep in mind that too much use of the first person can actually distract the reader from the main points [i.e., I know you're telling me this--just tell me!].

Analyzing vs. Summarizing. Department of English Writing Guide. George Mason University; Discussion. The Structure, Format, Content, and Style of a Journal-Style Scientific Paper. Department of Biology. Bates College; Hess, Dean R. "How to Write an Effective Discussion." Respiratory Care 49 (October 2004); Kretchmer, Paul. Fourteen Steps to Writing to Writing an Effective Discussion Section. San Francisco Edit, 2003-2008; The Lab Report. University College Writing Centre. University of Toronto; Sauaia, A. et al. "The Anatomy of an Article: The Discussion Section: "How Does the Article I Read Today Change What I Will Recommend to my Patients Tomorrow?” The Journal of Trauma and Acute Care Surgery 74 (June 2013): 1599-1602; Research Limitations & Future Research . Lund Research Ltd., 2012; Summary: Using it Wisely. The Writing Center. University of North Carolina; Schafer, Mickey S. Writing the Discussion. Writing in Psychology course syllabus. University of Florida; Yellin, Linda L. A Sociology Writer's Guide . Boston, MA: Allyn and Bacon, 2009.

Writing Tip

Don’t Over-Interpret the Results!

Interpretation is a subjective exercise. As such, you should always approach the selection and interpretation of your findings introspectively and to think critically about the possibility of judgmental biases unintentionally entering into discussions about the significance of your work. With this in mind, be careful that you do not read more into the findings than can be supported by the evidence you have gathered. Remember that the data are the data: nothing more, nothing less.

MacCoun, Robert J. "Biases in the Interpretation and Use of Research Results." Annual Review of Psychology 49 (February 1998): 259-287; Ward, Paulet al, editors. The Oxford Handbook of Expertise . Oxford, UK: Oxford University Press, 2018.

Another Writing Tip

Don't Write Two Results Sections!

One of the most common mistakes that you can make when discussing the results of your study is to present a superficial interpretation of the findings that more or less re-states the results section of your paper. Obviously, you must refer to your results when discussing them, but focus on the interpretation of those results and their significance in relation to the research problem, not the data itself.

Azar, Beth. "Discussing Your Findings."  American Psychological Association gradPSYCH Magazine (January 2006).

Yet Another Writing Tip

Avoid Unwarranted Speculation!

The discussion section should remain focused on the findings of your study. For example, if the purpose of your research was to measure the impact of foreign aid on increasing access to education among disadvantaged children in Bangladesh, it would not be appropriate to speculate about how your findings might apply to populations in other countries without drawing from existing studies to support your claim or if analysis of other countries was not a part of your original research design. If you feel compelled to speculate, do so in the form of describing possible implications or explaining possible impacts. Be certain that you clearly identify your comments as speculation or as a suggestion for where further research is needed. Sometimes your professor will encourage you to expand your discussion of the results in this way, while others don’t care what your opinion is beyond your effort to interpret the data in relation to the research problem.

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Discussion Section Examples and Writing Tips

Abstract | Introduction | Literature Review | Research question | Materials & Methods | Results | Discussion | Conclusion

In this blog, we look at how to write the discussion section of a research paper. We will go through plenty of discussion examples and understand how to construct a great discussion section for your research paper.

1. What is the purpose of the discussion section?

The discussion section is one of the most important sections of your research paper. This is where you interpret your results, highlight your contributions, and explain the value of your work to your readers.  This is one of the challenging parts to write because the author must clearly explain the significance of their results and tie everything back to the research questions.

2. How should I structure my discussion section?

Generally, the discussion section of a research paper typically contains the following parts.

Research summary It is a good idea to start this section with an overall summary of your work and highlight the main findings of your research.

Interpretation of findings You must interpret your findings clearly to your readers one by one.

Comparison with literature You must talk about how your results fit into existing research in the literature.

Implications of your work You should talk about the implications and possible benefits of your research.

Limitations You should talk about the possible limitations and shortcomings of your research

Future work And finally, you can talk about the possible future directions of your work.

3. Discussion Examples

Let’s look at some examples of the discussion section.  We will be looking at discussion examples from different fields and of different formats. We have split this section into multiple components so that it is easy for you to digest and understand.

3.1. An example of research summary in discussion

It is a good idea to start your discussion section with the summary of your work. The best way to do this will be to restate your research question, and then reminding your readers about your methods, and finally providing an overall summary of your results.

Our aims were to compare the effectiveness and user-friendliness of different storm detection software for storm tracking. On the basis of these aims, we ran multiple experiments with the same conditions using different storm detection software. Our results showed that in both speed and accuracy of data, ‘software A’ performed better than ‘software B’. _  Aims summary  _  Methodology summary  _  Results summary

This discussion example is from an engineering research paper. The authors are restating their aims first, which is to compare different types of storm-tracking software. Then, they are providing a brief summary of the methods. Here, they are testing different storm-tracking software under different conditions to see which performs the best. Then, they are finally providing their main finding which is that they found ‘software A’ better than ‘software B’.  This is a very good example of how to start the discussion section by presenting a summary of your work.

3.2. An example of result interpretation in discussion

The next step is to interpret your results. You have to explain your results clearly to your readers. Here is a discussion example that shows how to interpret your results.

The results of this study indicate significant differences between classical music and pop music in terms of their effects on memory recall and cognition. This implies that as the complexity of the music increases, so does its ability to facilitate cognitive processing. This finding aligns with the well-known “Mozart effect,” which suggests that listening to classical music can enhance cognitive function. _  Result  _  Interpretation  _   Additional evidence

The authors are saying that their results show that there is a significant difference between pop music and classical music in terms of memory recall and cognition. Now they are providing their interpretation of the findings. They think it is because there is a link between the complexity of music and cognitive processing. They are also making a reference to a well-known theory called the ‘Mozart effect’ to back up their findings. It is a nicely written passage and the author’s interpretation sounds very convincing and credible.

3.3. An example of literature comparison in discussion

The next step is to compare your results to the literature. You have to explain clearly how your findings compare with similar findings made by other researchers. Here is a discussion example where authors are providing details of papers in the literature that both support and oppose their findings.

Our analysis predicts that climate change will have a significant impact on wheat yield. This finding undermines one of the central pieces of evidence in some previous simulation studies [1-3] that suggest a negative effect of climate change on wheat yield, but the result is entirely consistent with the predictions of other research [4-5] that suggests the overall change in climate could result in increases in wheat yield. _  Result  _  Comparison with literature

The authors are saying that their results show that climate change will have a significant effect on wheat production. Then, they are saying that there are some papers in the literature that are in agreement with their findings. However, there are also many papers in the literature that disagree with their findings. This is very important. Your discussion should be two-sided, not one-sided. You should not ignore the literature that doesn’t corroborate your findings.

3.4. An example of research implications in discussion

The next step is to explain to your readers how your findings will benefit society and the research community. You have to clearly explain the value of your work to your readers. Here is a discussion example where authors explain the implications of their research.

The results contribute insights with regard to the management of wildfire events using artificial intelligence. One could easily argue that the obvious practical implication of this study is that it proposes utilizing cloud-based machine vision to detect wildfires in real-time, even before the first responders receive emergency calls. _  Your finding  _  Implications of your finding

In this paper, the authors are saying that their findings indicate that Artificial intelligence can be used to effectively manage wildfire events. Then, they are talking about the practical implications of their study. They are saying that their work has proven that machine learning can be used to detect wildfires in real-time. This is a great practical application and can save thousands of lives. As you can see, after reading this passage, you can immediately understand the value and significance of the work.

3.5. An example of limitations in discussion

It is very important that you discuss the limitations of your study. Limitations are flaws and shortcomings of your study. You have to tell your readers how your limitations might influence the outcomes and conclusions of your research. Most studies will have some form of limitation. So be honest and don’t hide your limitations. In reality, your readers and reviewers will be impressed with your paper if you are upfront about your limitations. 

Study design and small sample size are important limitations. This could have led to an overestimation of the effect. Future research should reconfirm these findings by conducting larger-scale studies. _  Limitation  _  How it might affect the results?  _   How to fix the limitation?

Here is a discussion example where the author talks about study limitations. The authors are saying that the main limitations of the study are the small sample size and weak study design. Then they explain how this might have affected their results. They are saying that it is possible that they are overestimating the actual effect they are measuring. Then finally they are telling the readers that more studies with larger sample sizes should be conducted to reconfirm the findings.

As you can see, the authors are clearly explaining three things here:

3.6. An example of future work in discussion

It is important to remember not to end your paper with limitations. Finish your paper on a positive note by telling your readers about the benefits of your research and possible future directions. Here is a discussion example where the author talks about future work.

Our study highlights useful insights about the potential of biomass as a renewable energy source. Future research can extend this research in several ways, including research on how to tackle challenges that hinder the sustainability of renewable energy sources towards climate change mitigation, such as market failures, lack of information and access to raw materials.   _  Benefits of your work  _   Future work

The authors are starting the final paragraph of the discussion section by highlighting the benefit of their work which is the use of biomass as a renewable source of energy. Then they talk about future research. They are saying that future research can focus on how to improve the sustainability of biomass production. This is a very good example of how to finish the discussion section of your paper on a positive note.

4. Frequently Asked Questions

Sometimes you will have negative or unexpected results in your paper. You have to talk about it in your discussion section. A lot of students find it difficult to write this part. The best way to handle this situation is not to look at results as either positive or negative. A result is a result, and you will always have something important and interesting to say about your findings. Just spend some time investigating what might have caused this result and tell your readers about it.

You must talk about the limitations of your work in the discussion section of the paper. One of the important qualities that the scientific community expects from a researcher is honesty and admitting when they have made a mistake. The important trick you have to learn while presenting your limitations is to present them in a constructive way rather than being too negative about them.  You must try to use positive language even when you are talking about major limitations of your work. 

If you have something exciting to say about your results or found something new that nobody else has found before, then, don’t be modest and use flat language when presenting this in the discussion. Use words like ‘break through’, ‘indisputable evidence’, ‘exciting proposition’ to increase the impact of your findings.

Important thing to remember is not to overstate your findings. If you found something really interesting but are not 100% sure, you must not mislead your readers. The best way to do this will be to use words like ‘it appears’ and ‘it seems’. This will tell the readers that there is a slight possibility that you might be wrong.

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Technical Terms, Notations, and Scientific Jargon in Research Papers

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6 Steps to Write an Excellent Discussion in Your Manuscript

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Table of Contents

The discussion section in scientific manuscripts might be the last few paragraphs, but its role goes far beyond wrapping up. It’s the part of an article where scientists talk about what they found and what it means, where raw data turns into meaningful insights. Therefore, discussion is a vital component of the article.  

An excellent discussion is well-organized. We bring to you authors a classic 6-step method for writing discussion sections, with examples to illustrate the functions and specific writing logic of each step. Take a look at how you can impress journal reviewers with a concise and focused discussion section!  

Discussion frame structure   

Conventionally, a discussion section has three parts: an introductory paragraph, a few intermediate paragraphs, and a conclusion¹.  Please follow the steps below:  

1.Introduction—mention gaps in previous research¹⁻ ²

Here, you orient the reader to your study. In the first paragraph, it is advisable to mention the research gap your paper addresses.  

Example: This study investigated the cognitive effects of a meat-only diet on adults. While earlier studies have explored the impact of a carnivorous diet on physical attributes and agility, they have not explicitly addressed its influence on cognitively intense tasks involving memory and reasoning.  

2. Summarizing key findings—let your data speak ¹⁻ ²

After you have laid out the context for your study, recapitulate some of its key findings. Also, highlight key data and evidence supporting these findings.  

Example: We found that risk-taking behavior among teenagers correlates with their tendency to invest in cryptocurrencies. Risk takers in this study, as measured by the Cambridge Gambling Task, tended to have an inordinately higher proportion of their savings invested as crypto coins.  

3. Interpreting results—compare with other papers¹⁻²    

Here, you must analyze and interpret any results concerning the research question or hypothesis. How do the key findings of your study help verify or disprove the hypothesis? What practical relevance does your discovery have?  

Example: Our study suggests that higher daily caffeine intake is not associated with poor performance in major sporting events. Athletes may benefit from the cardiovascular benefits of daily caffeine intake without adversely impacting performance.    

Remember, unlike the results section, the discussion ideally focuses on locating your findings in the larger body of existing research. Hence, compare your results with those of other peer-reviewed papers.  

Example: Although Miller et al. (2020) found evidence of such political bias in a multicultural population, our findings suggest that the bias is weak or virtually non-existent among politically active citizens.  

4. Addressing limitations—their potential impact on the results¹⁻²    

Discuss the potential impact of limitations on the results. Most studies have limitations, and it is crucial to acknowledge them in the intermediary paragraphs of the discussion section. Limitations may include low sample size, suspected interference or noise in data, low effect size, etc.  

Example: This study explored a comprehensive list of adverse effects associated with the novel drug ‘X’. However, long-term studies may be needed to confirm its safety, especially regarding major cardiac events.  

5. Implications for future research—how to explore further¹⁻²    

Locate areas of your research where more investigation is needed. Concluding paragraphs of the discussion can explain what research will likely confirm your results or identify knowledge gaps your study left unaddressed.  

Example: Our study demonstrates that roads paved with the plastic-infused compound ‘Y’ are more resilient than asphalt. Future studies may explore economically feasible ways of producing compound Y in bulk.  

6. Conclusion—summarize content¹⁻²    

A good way to wind up the discussion section is by revisiting the research question mentioned in your introduction. Sign off by expressing the main findings of your study.  

Example: Recent observations suggest that the fish ‘Z’ is moving upriver in many parts of the Amazon basin. Our findings provide conclusive evidence that this phenomenon is associated with rising sea levels and climate change, not due to elevated numbers of invasive predators.  

A rigorous and concise discussion section is one of the keys to achieving an excellent paper. It serves as a critical platform for researchers to interpret and connect their findings with the broader scientific context. By detailing the results, carefully comparing them with existing research, and explaining the limitations of this study, you can effectively help reviewers and readers understand the entire research article more comprehensively and deeply¹⁻² , thereby helping your manuscript to be successfully published and gain wider dissemination.  

In addition to keeping this writing guide, you can also use Elsevier Language Services to improve the quality of your paper more deeply and comprehensively. We have a professional editing team covering multiple disciplines. With our profound disciplinary background and rich polishing experience, we can significantly optimize all paper modules including the discussion, effectively improve the fluency and rigor of your articles, and make your scientific research results consistent, with its value reflected more clearly. We are always committed to ensuring the quality of papers according to the standards of top journals, improving the publishing efficiency of scientific researchers, and helping you on the road to academic success. Check us out here !  

Type in wordcount for Standard Total: USD EUR JPY Follow this link if your manuscript is longer than 12,000 words. Upload  

References:   

• Masic, I. (2018). How to write an efficient discussion? Medical Archives , 72(3), 306. https://doi.org/10.5455/medarh.2018.72.306-307  
• Şanlı, Ö., Erdem, S., & Tefik, T. (2014). How to write a discussion section? Urology Research & Practice , 39(1), 20–24. https://doi.org/10.5152/tud.2013.049  

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How to Write the Discussion Section of a Research Paper

The discussion section of a research paper analyzes and interprets the findings, provides context, compares them with previous studies, identifies limitations, and suggests future research directions.

Updated on September 15, 2023

Structure your discussion section right, and you’ll be cited more often while doing a greater service to the scientific community. So, what actually goes into the discussion section? And how do you write it?

The discussion section of your research paper is where you let the reader know how your study is positioned in the literature, what to take away from your paper, and how your work helps them. It can also include your conclusions and suggestions for future studies.

First, we’ll define all the parts of your discussion paper, and then look into how to write a strong, effective discussion section for your paper or manuscript.

Discussion section: what is it, what it does

The discussion section comes later in your paper, following the introduction, methods, and results. The discussion sets up your study’s conclusions. Its main goals are to present, interpret, and provide a context for your results.

What is it?

The discussion section provides an analysis and interpretation of the findings, compares them with previous studies, identifies limitations, and suggests future directions for research.

This section combines information from the preceding parts of your paper into a coherent story. By this point, the reader already knows why you did your study (introduction), how you did it (methods), and what happened (results). In the discussion, you’ll help the reader connect the ideas from these sections.

Why is it necessary?

The discussion provides context and interpretations for the results. It also answers the questions posed in the introduction. While the results section describes your findings, the discussion explains what they say. This is also where you can describe the impact or implications of your research.

Adds context for your results

Most research studies aim to answer a question, replicate a finding, or address limitations in the literature. These goals are first described in the introduction. However, in the discussion section, the author can refer back to them to explain how the study's objective was achieved. 

Shows what your results actually mean and real-world implications

The discussion can also describe the effect of your findings on research or practice. How are your results significant for readers, other researchers, or policymakers?

What to include in your discussion (in the correct order)

A complete and effective discussion section should at least touch on the points described below.

Summary of key findings

The discussion should begin with a brief factual summary of the results. Concisely overview the main results you obtained.

Begin with key findings with supporting evidence

Your results section described a list of findings, but what message do they send when you look at them all together?

Your findings were detailed in the results section, so there’s no need to repeat them here, but do provide at least a few highlights. This will help refresh the reader’s memory and help them focus on the big picture.

Read the first paragraph of the discussion section in this article (PDF) for an example of how to start this part of your paper. Notice how the authors break down their results and follow each description sentence with an explanation of why each finding is relevant. 

State clearly and concisely

Following a clear and direct writing style is especially important in the discussion section. After all, this is where you will make some of the most impactful points in your paper. While the results section often contains technical vocabulary, such as statistical terms, the discussion section lets you describe your findings more clearly. 

Interpretation of results

Once you’ve given your reader an overview of your results, you need to interpret those results. In other words, what do your results mean? Discuss the findings’ implications and significance in relation to your research question or hypothesis.

Analyze and interpret your findings

Look into your findings and explore what’s behind them or what may have caused them. If your introduction cited theories or studies that could explain your findings, use these sources as a basis to discuss your results.

For example, look at the second paragraph in the discussion section of this article on waggling honey bees. Here, the authors explore their results based on information from the literature.

Unexpected or contradictory results

Sometimes, your findings are not what you expect. Here’s where you describe this and try to find a reason for it. Could it be because of the method you used? Does it have something to do with the variables analyzed? Comparing your methods with those of other similar studies can help with this task.

Context and comparison with previous work

Refer to related studies to place your research in a larger context and the literature. Compare and contrast your findings with existing literature, highlighting similarities, differences, and/or contradictions.

How your work compares or contrasts with previous work

Studies with similar findings to yours can be cited to show the strength of your findings. Information from these studies can also be used to help explain your results. Differences between your findings and others in the literature can also be discussed here. 

How to divide this section into subsections

If you have more than one objective in your study or many key findings, you can dedicate a separate section to each of these. Here’s an example of this approach. You can see that the discussion section is divided into topics and even has a separate heading for each of them. 

Limitations

Many journals require you to include the limitations of your study in the discussion. Even if they don’t, there are good reasons to mention these in your paper.

Why limitations don’t have a negative connotation

A study’s limitations are points to be improved upon in future research. While some of these may be flaws in your method, many may be due to factors you couldn’t predict.

Examples include time constraints or small sample sizes. Pointing this out will help future researchers avoid or address these issues. This part of the discussion can also include any attempts you have made to reduce the impact of these limitations, as in this study .

How limitations add to a researcher's credibility

Pointing out the limitations of your study demonstrates transparency. It also shows that you know your methods well and can conduct a critical assessment of them.  

Implications and significance

The final paragraph of the discussion section should contain the take-home messages for your study. It can also cite the “strong points” of your study, to contrast with the limitations section.

Restate your hypothesis

Remind the reader what your hypothesis was before you conducted the study. 

How was it proven or disproven?

Identify your main findings and describe how they relate to your hypothesis.

How your results contribute to the literature

Were you able to answer your research question? Or address a gap in the literature?

Future implications of your research

Describe the impact that your results may have on the topic of study. Your results may show, for instance, that there are still limitations in the literature for future studies to address. There may be a need for studies that extend your findings in a specific way. You also may need additional research to corroborate your findings. 

Sample discussion section

This fictitious example covers all the aspects discussed above. Your actual discussion section will probably be much longer, but you can read this to get an idea of everything your discussion should cover.

Our results showed that the presence of cats in a household is associated with higher levels of perceived happiness by its human occupants. These findings support our hypothesis and demonstrate the association between pet ownership and well-being. 

The present findings align with those of Bao and Schreer (2016) and Hardie et al. (2023), who observed greater life satisfaction in pet owners relative to non-owners. Although the present study did not directly evaluate life satisfaction, this factor may explain the association between happiness and cat ownership observed in our sample.

Our findings must be interpreted in light of some limitations, such as the focus on cat ownership only rather than pets as a whole. This may limit the generalizability of our results.

Nevertheless, this study had several strengths. These include its strict exclusion criteria and use of a standardized assessment instrument to investigate the relationships between pets and owners. These attributes bolster the accuracy of our results and reduce the influence of confounding factors, increasing the strength of our conclusions. Future studies may examine the factors that mediate the association between pet ownership and happiness to better comprehend this phenomenon.

This brief discussion begins with a quick summary of the results and hypothesis. The next paragraph cites previous research and compares its findings to those of this study. Information from previous studies is also used to help interpret the findings. After discussing the results of the study, some limitations are pointed out. The paper also explains why these limitations may influence the interpretation of results. Then, final conclusions are drawn based on the study, and directions for future research are suggested.

How to make your discussion flow naturally

If you find writing in scientific English challenging, the discussion and conclusions are often the hardest parts of the paper to write. That’s because you’re not just listing up studies, methods, and outcomes. You’re actually expressing your thoughts and interpretations in words.

• How formal should it be?
• What words should you use, or not use?
• How do you meet strict word limits, or make it longer and more informative?

Always give it your best, but sometimes a helping hand can, well, help. Getting a professional edit can help clarify your work’s importance while improving the English used to explain it. When readers know the value of your work, they’ll cite it. We’ll assign your study to an expert editor knowledgeable in your area of research. Their work will clarify your discussion, helping it to tell your story. Find out more about AJE Editing.

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How to Write a Discussion Section for a Research Paper

We’ve talked about several useful writing tips that authors should consider while drafting or editing their research papers. In particular, we’ve focused on  figures and legends , as well as the Introduction ,  Methods , and  Results . Now that we’ve addressed the more technical portions of your journal manuscript, let’s turn to the analytical segments of your research article. In this article, we’ll provide tips on how to write a strong Discussion section that best portrays the significance of your research contributions.

What is the Discussion section of a research paper?

In a nutshell,  your Discussion fulfills the promise you made to readers in your Introduction . At the beginning of your paper, you tell us why we should care about your research. You then guide us through a series of intricate images and graphs that capture all the relevant data you collected during your research. We may be dazzled and impressed at first, but none of that matters if you deliver an anti-climactic conclusion in the Discussion section!

Are you feeling pressured? Don’t worry. To be honest, you will edit the Discussion section of your manuscript numerous times. After all, in as little as one to two paragraphs ( Nature ‘s suggestion  based on their 3,000-word main body text limit), you have to explain how your research moves us from point A (issues you raise in the Introduction) to point B (our new understanding of these matters). You must also recommend how we might get to point C (i.e., identify what you think is the next direction for research in this field). That’s a lot to say in two paragraphs!

So, how do you do that? Let’s take a closer look.

What should I include in the Discussion section?

As we stated above, the goal of your Discussion section is to  answer the questions you raise in your Introduction by using the results you collected during your research . The content you include in the Discussions segment should include the following information:

• Remind us why we should be interested in this research project.
• Describe the nature of the knowledge gap you were trying to fill using the results of your study.
• Don’t repeat your Introduction. Instead, focus on why  this  particular study was needed to fill the gap you noticed and why that gap needed filling in the first place.
• Mainly, you want to remind us of how your research will increase our knowledge base and inspire others to conduct further research.
• Clearly tell us what that piece of missing knowledge was.
• Answer each of the questions you asked in your Introduction and explain how your results support those conclusions.
• Make sure to factor in all results relevant to the questions (even if those results were not statistically significant).
• Focus on the significance of the most noteworthy results.
• If conflicting inferences can be drawn from your results, evaluate the merits of all of them.
• Don’t rehash what you said earlier in the Results section. Rather, discuss your findings in the context of answering your hypothesis. Instead of making statements like “[The first result] was this…,” say, “[The first result] suggests [conclusion].”
• Do your conclusions line up with existing literature?
• Discuss whether your findings agree with current knowledge and expectations.
• Keep in mind good persuasive argument skills, such as explaining the strengths of your arguments and highlighting the weaknesses of contrary opinions.
• If you discovered something unexpected, offer reasons. If your conclusions aren’t aligned with current literature, explain.
• Address any limitations of your study and how relevant they are to interpreting your results and validating your findings.
• Make sure to acknowledge any weaknesses in your conclusions and suggest room for further research concerning that aspect of your analysis.
• Make sure your suggestions aren’t ones that should have been conducted during your research! Doing so might raise questions about your initial research design and protocols.
• Similarly, maintain a critical but unapologetic tone. You want to instill confidence in your readers that you have thoroughly examined your results and have objectively assessed them in a way that would benefit the scientific community’s desire to expand our knowledge base.
• Recommend next steps.
• Your suggestions should inspire other researchers to conduct follow-up studies to build upon the knowledge you have shared with them.
• Keep the list short (no more than two).

How to Write the Discussion Section

The above list of what to include in the Discussion section gives an overall idea of what you need to focus on throughout the section. Below are some tips and general suggestions about the technical aspects of writing and organization that you might find useful as you draft or revise the contents we’ve outlined above.

Technical writing elements

• Embrace active voice because it eliminates the awkward phrasing and wordiness that accompanies passive voice.
• Use the present tense, which should also be employed in the Introduction.
• Sprinkle with first person pronouns if needed, but generally, avoid it. We want to focus on your findings.
• Maintain an objective and analytical tone.

Discussion section organization

• Keep the same flow across the Results, Methods, and Discussion sections.
• We develop a rhythm as we read and parallel structures facilitate our comprehension. When you organize information the same way in each of these related parts of your journal manuscript, we can quickly see how a certain result was interpreted and quickly verify the particular methods used to produce that result.
• Notice how using parallel structure will eliminate extra narration in the Discussion part since we can anticipate the flow of your ideas based on what we read in the Results segment. Reducing wordiness is important when you only have a few paragraphs to devote to the Discussion section!
• Within each subpart of a Discussion, the information should flow as follows: (A) conclusion first, (B) relevant results and how they relate to that conclusion and (C) relevant literature.
• End with a concise summary explaining the big-picture impact of your study on our understanding of the subject matter. At the beginning of your Discussion section, you stated why  this  particular study was needed to fill the gap you noticed and why that gap needed filling in the first place. Now, it is time to end with “how your research filled that gap.”

Discussion Part 1: Summarizing Key Findings

Begin the Discussion section by restating your  statement of the problem  and briefly summarizing the major results. Do not simply repeat your findings. Rather, try to create a concise statement of the main results that directly answer the central research question that you stated in the Introduction section . This content should not be longer than one paragraph in length.

Many researchers struggle with understanding the precise differences between a Discussion section and a Results section . The most important thing to remember here is that your Discussion section should subjectively evaluate the findings presented in the Results section, and in relatively the same order. Keep these sections distinct by making sure that you do not repeat the findings without providing an interpretation.

Phrase examples: Summarizing the results

• The findings indicate that …
• These results suggest a correlation between A and B …
• The data present here suggest that …
• An interpretation of the findings reveals a connection between…

Discussion Part 2: Interpreting the Findings

What do the results mean? It may seem obvious to you, but simply looking at the figures in the Results section will not necessarily convey to readers the importance of the findings in answering your research questions.

The exact structure of interpretations depends on the type of research being conducted. Here are some common approaches to interpreting data:

• Identifying correlations and relationships in the findings
• Explaining whether the results confirm or undermine your research hypothesis
• Giving the findings context within the history of similar research studies
• Discussing unexpected results and analyzing their significance to your study or general research
• Offering alternative explanations and arguing for your position

Organize the Discussion section around key arguments, themes, hypotheses, or research questions or problems. Again, make sure to follow the same order as you did in the Results section.

Discussion Part 3: Discussing the Implications

In addition to providing your own interpretations, show how your results fit into the wider scholarly literature you surveyed in the  literature review section. This section is called the implications of the study . Show where and how these results fit into existing knowledge, what additional insights they contribute, and any possible consequences that might arise from this knowledge, both in the specific research topic and in the wider scientific domain.

Questions to ask yourself when dealing with potential implications:

• Do your findings fall in line with existing theories, or do they challenge these theories or findings? What new information do they contribute to the literature, if any? How exactly do these findings impact or conflict with existing theories or models?
• What are the practical implications on actual subjects or demographics?
• What are the methodological implications for similar studies conducted either in the past or future?

Your purpose in giving the implications is to spell out exactly what your study has contributed and why researchers and other readers should be interested.

Phrase examples: Discussing the implications of the research

• These results confirm the existing evidence in X studies…
• The results are not in line with the foregoing theory that…
• This experiment provides new insights into the connection between…
• These findings present a more nuanced understanding of…
• While previous studies have focused on X, these results demonstrate that Y.

Step 4: Acknowledging the limitations

All research has study limitations of one sort or another. Acknowledging limitations in methodology or approach helps strengthen your credibility as a researcher. Study limitations are not simply a list of mistakes made in the study. Rather, limitations help provide a more detailed picture of what can or cannot be concluded from your findings. In essence, they help temper and qualify the study implications you listed previously.

Study limitations can relate to research design, specific methodological or material choices, or unexpected issues that emerged while you conducted the research. Mention only those limitations directly relate to your research questions, and explain what impact these limitations had on how your study was conducted and the validity of any interpretations.

Possible types of study limitations:

• Insufficient sample size for statistical measurements
• Lack of previous research studies on the topic
• Methods/instruments/techniques used to collect the data
• Limited access to data
• Time constraints in properly preparing and executing the study

After discussing the study limitations, you can also stress that your results are still valid. Give some specific reasons why the limitations do not necessarily handicap your study or narrow its scope.

Phrase examples: Limitations sentence beginners

• “There may be some possible limitations in this study.”
• “The findings of this study have to be seen in light of some limitations.”
•  “The first limitation is the…The second limitation concerns the…”
•  “The empirical results reported herein should be considered in the light of some limitations.”
• “This research, however, is subject to several limitations.”
• “The primary limitation to the generalization of these results is…”
• “Nonetheless, these results must be interpreted with caution and a number of limitations should be borne in mind.”

Discussion Part 5: Giving Recommendations for Further Research

Based on your interpretation and discussion of the findings, your recommendations can include practical changes to the study or specific further research to be conducted to clarify the research questions. Recommendations are often listed in a separate Conclusion section , but often this is just the final paragraph of the Discussion section.

Suggestions for further research often stem directly from the limitations outlined. Rather than simply stating that “further research should be conducted,” provide concrete specifics for how future can help answer questions that your research could not.

Phrase examples: Recommendation sentence beginners

• Further research is needed to establish …
• There is abundant space for further progress in analyzing…
• A further study with more focus on X should be done to investigate…
• Further studies of X that account for these variables must be undertaken.

Consider Receiving Professional Language Editing

As you edit or draft your research manuscript, we hope that you implement these guidelines to produce a more effective Discussion section. And after completing your draft, don’t forget to submit your work to a professional proofreading and English editing service like Wordvice, including our manuscript editing service for  paper editing , cover letter editing , SOP editing , and personal statement proofreading services. Language editors not only proofread and correct errors in grammar, punctuation, mechanics, and formatting but also improve terms and revise phrases so they read more naturally. Wordvice is an industry leader in providing high-quality revision for all types of academic documents.

For additional information about how to write a strong research paper, make sure to check out our full  research writing series !

Wordvice Writing Resources

• How to Write a Research Paper Introduction 
• Which Verb Tenses to Use in a Research Paper
• How to Write an Abstract for a Research Paper
• How to Write a Research Paper Title
• Useful Phrases for Academic Writing
• Common Transition Terms in Academic Papers
• Active and Passive Voice in Research Papers
• 100+ Verbs That Will Make Your Research Writing Amazing
• Tips for Paraphrasing in Research Papers

Additional Academic Resources

•   Guide for Authors.  (Elsevier)
•  How to Write the Results Section of a Research Paper.  (Bates College)
•   Structure of a Research Paper.  (University of Minnesota Biomedical Library)
•   How to Choose a Target Journal  (Springer)
•   How to Write Figures and Tables  (UNC Writing Center)

• Research Paper Guides
• Basics of Research Paper Writing

How to Write a Discussion Section: Writing Guide

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Table of contents

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The discussion section of a research paper is where the author analyzes and explains the importance of the study's results. It presents the conclusions drawn from the study, compares them to previous research, and addresses any potential limitations or weaknesses. The discussion section should also suggest areas for future research.

Everything is not that complicated if you know where to find the required information. We’ll tell you everything there is to know about writing your discussion. Our easy guide covers all important bits, including research questions and your research results. Do you know how all enumerated events are connected? Well, you will after reading this guide we’ve prepared for you!

What Is in the Discussion Section of a Research Paper

The discussion section of a research paper can be viewed as something similar to the conclusion of your paper. But not literal, of course. It’s an ultimate section where you can talk about the findings of your study. Think about these questions when writing:

• Did you answer all of the promised research questions?
• Did you mention why your work matters?
• What are your findings, and why should anyone even care?
• Does your study have a literature review?

So, answer your questions, provide proof, and don’t forget about your promises from the introduction. 

How to Write a Discussion Section in 5 Steps

How to write the discussion section of a research paper is something everyone googles eventually. It's just life. But why not make everything easier? In brief, this section we’re talking about must include all following parts:

• Answers for research questions
• Literature review
• Results of the work
• Limitations of one’s study
• Overall conclusion

Indeed, all those parts may confuse anyone. So by looking at our guide, you'll save yourself some hassle.  P.S. All our steps are easy and explained in detail! But if you are looking for the most efficient solution, consider using professional help. Leave your “ write my research paper for me ” order at StudyCrumb and get a customized study tailored to your requirements.

Step 1. Start Strong: Discussion Section of a Research Paper

First and foremost, how to start the discussion section of a research paper? Here’s what you should definitely consider before settling down to start writing:

• All essays or papers must begin strong. All readers will not wait for any writer to get to the point. We advise summarizing the paper's main findings.
• Moreover, you should relate both discussion and literature review to what you have discovered. Mentioning that would be a plus too.
• Make sure that an introduction or start per se is clear and concise. Word count might be needed for school. But any paper should be understandable and not too diluted.

Step 2. Answer the Questions in Your Discussion Section of a Research Paper

Writing the discussion section of a research paper also involves mentioning your questions. Remember that in your introduction, you have promised your readers to answer certain questions. Well, now it’s a perfect time to finally give the awaited answer. You need to explain all possible correlations between your findings, research questions, and literature proposed. You already had hypotheses. So were they correct, or maybe you want to propose certain corrections? Section’s main goal is to avoid open ends. It’s not a story or a fairytale with an intriguing ending. If you have several questions, you must answer them. As simple as that.

Step 3. Relate Your Results in a Discussion Section

Writing a discussion section of a research paper also requires any writer to explain their results. You will undoubtedly include an impactful literature review. However, your readers should not just try and struggle with understanding what are some specific relationships behind previous studies and your results.  Your results should sound something like: “This guy in their paper discovered that apples are green. Nevertheless, I have proven via experimentation and research that apples are actually red.” Please, don’t take these results directly. It’s just an initial hypothesis. But what you should definitely remember is any practical implications of your study. Why does it matter and how can anyone use it? That’s the most crucial question.

Step 4. Describe the Limitations in Your Discussion Section

Discussion section of a research paper isn’t limitless. What does that mean? Essentially, it means that you also have to discuss any limitations of your study. Maybe you had some methodological inconsistencies. Possibly, there are no particular theories or not enough information for you to be entirely confident in one’s conclusions.  You might say that an available source of literature you have studied does not focus on one’s issue. That’s why one’s main limitation is theoretical. However, keep in mind that your limitations must possess a certain degree of relevancy. You can just say that you haven’t found enough books. Your information must be truthful to research.

Step 5. Conclude Your Discussion Section With Recommendations

Your last step when you write a discussion section in a paper is its conclusion, like in any other academic work. Writer’s conclusion must be as strong as their starting point of the overall work. Check out our brief list of things to know about the conclusion in research paper :

• It must present its scientific relevance and importance of your work.
• It should include different implications of your research.
• It should not, however, discuss anything new or things that you have not mentioned before.
• Leave no open questions and carefully complete the work without them.

Discussion Section of a Research Paper Example

All the best example discussion sections of a research paper will be written according to our brief guide. Don’t forget that you need to state your findings and underline the importance of your work. An undoubtedly big part of one’s discussion will definitely be answering and explaining the research questions. In other words, you’ll already have all the knowledge you have so carefully gathered. Our last step for you is to recollect and wrap up your paper. But we’re sure you’ll succeed!

How to Write a Discussion Section: Final Thoughts

Today we have covered how to write a discussion section. That was quite a brief journey, wasn’t it? Just to remind you to focus on these things:

• Importance of your study.
• Summary of the information you have gathered.
• Main findings and conclusions.
• Answers to all research questions without an open end.
• Correlation between literature review and your results.

But, wait, this guide is not the only thing we can do. Looking for how to write an abstract for a research paper  for example? We have such a blog and much more on our platform.

Our academic writing service is just a click away. We are proud to say that our writers are professionals in their fields. Buy a research paper and our experts can provide prompt solutions without compromising the quality.

Discussion Section of a Research Paper: Frequently Asked Questions

1. how long should the discussion section of a research paper be.

Our discussion section of a research paper should not be longer than other sections. So try to keep it short but as informative as possible. It usually contains around 6-7 paragraphs in length. It is enough to briefly summarize all the important data and not to drag it.

2. What's the difference between the discussion and the results?

The difference between discussion and results is very simple and easy to understand. The results only report your main findings. You stated what you have found and how you have done that. In contrast, one’s discussion mentions your findings and explains how they relate to other literature, research questions, and one’s hypothesis. Therefore, it is not only a report but an efficient as well as proper explanation.

3. What's the difference between a discussion and a conclusion?

The difference between discussion and conclusion is also quite easy. Conclusion is a brief summary of all the findings and results. Still, our favorite discussion section interprets and explains your main results. It is an important but more lengthy and wordy part. Besides, it uses extra literature for references.

4. What is the purpose of the discussion section?

The primary purpose of a discussion section is to interpret and describe all your interesting findings. Therefore, you should state what you have learned, whether your hypothesis was correct and how your results can be explained using other sources. If this section is clear to readers, our congratulations as you have succeeded.

Joe Eckel is an expert on Dissertations writing. He makes sure that each student gets precious insights on composing A-grade academic writing.

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How to Start a Discussion Section in Research? [with Examples]

The examples below are from 72,017 full-text PubMed research papers that I analyzed in order to explore common ways to start writing the Discussion section.

Research papers included in this analysis were selected at random from those uploaded to PubMed Central between the years 2016 and 2021. Note that I used the BioC API to download the data (see the References section below).

Examples of how to start writing the Discussion section

In the Discussion section, you should explain the meaning of your results, their importance, and implications. [for more information, see: How to Write & Publish a Research Paper: Step-by-Step Guide ]

The Discussion section can:

1. Start by restating the study objective

“ The purpose of this study was to investigate the relationship between muscle synergies and motion primitives of the upper limb motions.” Taken from the Discussion section of this article on PubMed

“ The main objective of this study was to identify trajectories of autonomy.” Taken from the Discussion section of this article on PubMed

“ In the present study, we investigated the whole brain regional homogeneity in patients with melancholic MDD and non-melancholic MDD at rest . “ Taken from the Discussion section of this article on PubMed

2. Start by mentioning the main finding

“ We found that autocracy and democracy have acted as peaks in an evolutionary landscape of possible modes of institutional arrangements.” Taken from the Discussion section of this article on PubMed

“ In this study, we demonstrated that the neural mechanisms of rhythmic movements and skilled movements are similar.” Taken from the Discussion section of this article on PubMed

“ The results of this study show that older adults are a diverse group concerning their activities on the Internet.” Taken from the Discussion section of this article on PubMed

3. Start by pointing out the strength of the study

“ To our knowledge, this investigation is by far the largest epidemiological study employing real-time PCR to study periodontal pathogens in subgingival plaque.” Taken from the Discussion section of this article on PubMed

“ This is the first human subject research using the endoscopic hemoglobin oxygen saturation imaging technology for patients with aero-digestive tract cancers or adenomas.” Taken from the Discussion section of this article on PubMed

“ In this work, we introduced a new real-time flow imaging method and systematically demonstrated its effectiveness with both flow phantom experiments and in vivo experiments.” Taken from the Discussion section of this article on PubMed

Most used words at the start of the Discussion

Here are the top 10 phrases used to start a discussion section in our dataset:

• Comeau DC, Wei CH, Islamaj Doğan R, and Lu Z. PMC text mining subset in BioC: about 3 million full text articles and growing,  Bioinformatics , btz070, 2019.

Further reading

• How Long Should the Discussion Section Be? Data from 61,517 Examples
• How to Write & Publish a Research Paper: Step-by-Step Guide
• “I” & “We” in Academic Writing: Examples from 9,830 Studies

How To Write The Discussion Chapter

A Simple Explainer With Examples + Free Template

By: Jenna Crossley (PhD) | Reviewed By: Dr. Eunice Rautenbach | August 2021

If you’re reading this, chances are you’ve reached the discussion chapter of your thesis or dissertation and are looking for a bit of guidance. Well, you’ve come to the right place ! In this post, we’ll unpack and demystify the typical discussion chapter in straightforward, easy to understand language, with loads of examples .

Overview: The Discussion Chapter

• What  the discussion chapter is
• What to include in your discussion
• How to write up your discussion
• A few tips and tricks to help you along the way
• Free discussion template

What (exactly) is the discussion chapter?

The discussion chapter is where you interpret and explain your results within your thesis or dissertation. This contrasts with the results chapter, where you merely present and describe the analysis findings (whether qualitative or quantitative ). In the discussion chapter, you elaborate on and evaluate your research findings, and discuss the significance and implications of your results .

In this chapter, you’ll situate your research findings in terms of your research questions or hypotheses and tie them back to previous studies and literature (which you would have covered in your literature review chapter). You’ll also have a look at how relevant and/or significant your findings are to your field of research, and you’ll argue for the conclusions that you draw from your analysis. Simply put, the discussion chapter is there for you to interact with and explain your research findings in a thorough and coherent manner.

What should I include in the discussion chapter?

First things first: in some studies, the results and discussion chapter are combined into one chapter .  This depends on the type of study you conducted (i.e., the nature of the study and methodology adopted), as well as the standards set by the university.  So, check in with your university regarding their norms and expectations before getting started. In this post, we’ll treat the two chapters as separate, as this is most common.

Basically, your discussion chapter should analyse , explore the meaning and identify the importance of the data you presented in your results chapter. In the discussion chapter, you’ll give your results some form of meaning by evaluating and interpreting them. This will help answer your research questions, achieve your research aims and support your overall conclusion (s). Therefore, you discussion chapter should focus on findings that are directly connected to your research aims and questions. Don’t waste precious time and word count on findings that are not central to the purpose of your research project.

As this chapter is a reflection of your results chapter, it’s vital that you don’t report any new findings . In other words, you can’t present claims here if you didn’t present the relevant data in the results chapter first.  So, make sure that for every discussion point you raise in this chapter, you’ve covered the respective data analysis in the results chapter. If you haven’t, you’ll need to go back and adjust your results chapter accordingly.

If you’re struggling to get started, try writing down a bullet point list everything you found in your results chapter. From this, you can make a list of everything you need to cover in your discussion chapter. Also, make sure you revisit your research questions or hypotheses and incorporate the relevant discussion to address these.  This will also help you to see how you can structure your chapter logically.

Need a helping hand?

How to write the discussion chapter

Now that you’ve got a clear idea of what the discussion chapter is and what it needs to include, let’s look at how you can go about structuring this critically important chapter. Broadly speaking, there are six core components that need to be included, and these can be treated as steps in the chapter writing process.

Step 1: Restate your research problem and research questions

The first step in writing up your discussion chapter is to remind your reader of your research problem , as well as your research aim(s) and research questions . If you have hypotheses, you can also briefly mention these. This “reminder” is very important because, after reading dozens of pages, the reader may have forgotten the original point of your research or been swayed in another direction. It’s also likely that some readers skip straight to your discussion chapter from the introduction chapter , so make sure that your research aims and research questions are clear.

Step 2: Summarise your key findings

Next, you’ll want to summarise your key findings from your results chapter. This may look different for qualitative and quantitative research , where qualitative research may report on themes and relationships, whereas quantitative research may touch on correlations and causal relationships. Regardless of the methodology, in this section you need to highlight the overall key findings in relation to your research questions.

Typically, this section only requires one or two paragraphs , depending on how many research questions you have. Aim to be concise here, as you will unpack these findings in more detail later in the chapter. For now, a few lines that directly address your research questions are all that you need.

Some examples of the kind of language you’d use here include:

• The data suggest that…
• The data support/oppose the theory that…
• The analysis identifies…

These are purely examples. What you present here will be completely dependent on your original research questions, so make sure that you are led by them .

Step 3: Interpret your results

Once you’ve restated your research problem and research question(s) and briefly presented your key findings, you can unpack your findings by interpreting your results. Remember: only include what you reported in your results section – don’t introduce new information.

From a structural perspective, it can be a wise approach to follow a similar structure in this chapter as you did in your results chapter. This would help improve readability and make it easier for your reader to follow your arguments. For example, if you structured you results discussion by qualitative themes, it may make sense to do the same here.

Alternatively, you may structure this chapter by research questions, or based on an overarching theoretical framework that your study revolved around. Every study is different, so you’ll need to assess what structure works best for you.

When interpreting your results, you’ll want to assess how your findings compare to those of the existing research (from your literature review chapter). Even if your findings contrast with the existing research, you need to include these in your discussion. In fact, those contrasts are often the most interesting findings . In this case, you’d want to think about why you didn’t find what you were expecting in your data and what the significance of this contrast is.

Here are a few questions to help guide your discussion:

• How do your results relate with those of previous studies ?
• If you get results that differ from those of previous studies, why may this be the case?
• What do your results contribute to your field of research?
• What other explanations could there be for your findings?

When interpreting your findings, be careful not to draw conclusions that aren’t substantiated . Every claim you make needs to be backed up with evidence or findings from the data (and that data needs to be presented in the previous chapter – results). This can look different for different studies; qualitative data may require quotes as evidence, whereas quantitative data would use statistical methods and tests. Whatever the case, every claim you make needs to be strongly backed up.

Step 4: Acknowledge the limitations of your study

The fourth step in writing up your discussion chapter is to acknowledge the limitations of the study. These limitations can cover any part of your study , from the scope or theoretical basis to the analysis method(s) or sample. For example, you may find that you collected data from a very small sample with unique characteristics, which would mean that you are unable to generalise your results to the broader population.

For some students, discussing the limitations of their work can feel a little bit self-defeating . This is a misconception, as a core indicator of high-quality research is its ability to accurately identify its weaknesses. In other words, accurately stating the limitations of your work is a strength, not a weakness . All that said, be careful not to undermine your own research. Tell the reader what limitations exist and what improvements could be made, but also remind them of the value of your study despite its limitations.

Step 5: Make recommendations for implementation and future research

Now that you’ve unpacked your findings and acknowledge the limitations thereof, the next thing you’ll need to do is reflect on your study in terms of two factors:

• The practical application of your findings
• Suggestions for future research

The first thing to discuss is how your findings can be used in the real world – in other words, what contribution can they make to the field or industry? Where are these contributions applicable, how and why? For example, if your research is on communication in health settings, in what ways can your findings be applied to the context of a hospital or medical clinic? Make sure that you spell this out for your reader in practical terms, but also be realistic and make sure that any applications are feasible.

The next discussion point is the opportunity for future research . In other words, how can other studies build on what you’ve found and also improve the findings by overcoming some of the limitations in your study (which you discussed a little earlier). In doing this, you’ll want to investigate whether your results fit in with findings of previous research, and if not, why this may be the case. For example, are there any factors that you didn’t consider in your study? What future research can be done to remedy this? When you write up your suggestions, make sure that you don’t just say that more research is needed on the topic, also comment on how the research can build on your study.

Step 6: Provide a concluding summary

Finally, you’ve reached your final stretch. In this section, you’ll want to provide a brief recap of the key findings – in other words, the findings that directly address your research questions . Basically, your conclusion should tell the reader what your study has found, and what they need to take away from reading your report.

When writing up your concluding summary, bear in mind that some readers may skip straight to this section from the beginning of the chapter.  So, make sure that this section flows well from and has a strong connection to the opening section of the chapter.

Tips and tricks for an A-grade discussion chapter

Now that you know what the discussion chapter is , what to include and exclude , and how to structure it , here are some tips and suggestions to help you craft a quality discussion chapter.

• When you write up your discussion chapter, make sure that you keep it consistent with your introduction chapter , as some readers will skip from the introduction chapter directly to the discussion chapter. Your discussion should use the same tense as your introduction, and it should also make use of the same key terms.
• Don’t make assumptions about your readers. As a writer, you have hands-on experience with the data and so it can be easy to present it in an over-simplified manner. Make sure that you spell out your findings and interpretations for the intelligent layman.
• Have a look at other theses and dissertations from your institution, especially the discussion sections. This will help you to understand the standards and conventions of your university, and you’ll also get a good idea of how others have structured their discussion chapters. You can also check out our chapter template .
• Avoid using absolute terms such as “These results prove that…”, rather make use of terms such as “suggest” or “indicate”, where you could say, “These results suggest that…” or “These results indicate…”. It is highly unlikely that a dissertation or thesis will scientifically prove something (due to a variety of resource constraints), so be humble in your language.
• Use well-structured and consistently formatted headings to ensure that your reader can easily navigate between sections, and so that your chapter flows logically and coherently.

If you have any questions or thoughts regarding this post, feel free to leave a comment below. Also, if you’re looking for one-on-one help with your discussion chapter (or thesis in general), consider booking a free consultation with one of our highly experienced Grad Coaches to discuss how we can help you.

Psst... there’s more!

This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

38 Comments

Thank you this is helpful!

This is very helpful to me… Thanks a lot for sharing this with us 😊

This has been very helpful indeed. Thank you.

This is actually really helpful, I just stumbled upon it. Very happy that I found it, thank you.

Me too! I was kinda lost on how to approach my discussion chapter. How helpful! Thanks a lot!

This is really good and explicit. Thanks

Thank you, this blog has been such a help.

Thank you. This is very helpful.

Dear sir/madame

Thanks a lot for this helpful blog. Really, it supported me in writing my discussion chapter while I was totally unaware about its structure and method of writing.

With regards

Syed Firoz Ahmad PhD, Research Scholar

I agree so much. This blog was god sent. It assisted me so much while I was totally clueless about the context and the know-how. Now I am fully aware of what I am to do and how I am to do it.

Thanks! This is helpful!

thanks alot for this informative website

Dear Sir/Madam,

Truly, your article was much benefited when i structured my discussion chapter.

Thank you very much!!!

This is helpful for me in writing my research discussion component. I have to copy this text on Microsoft word cause of my weakness that I cannot be able to read the text on screen a long time. So many thanks for this articles.

This was helpful

Thanks Jenna, well explained.

Thank you! This is super helpful.

Thanks very much. I have appreciated the six steps on writing the Discussion chapter which are (i) Restating the research problem and questions (ii) Summarising the key findings (iii) Interpreting the results linked to relating to previous results in positive and negative ways; explaining whay different or same and contribution to field of research and expalnation of findings (iv) Acknowledgeing limitations (v) Recommendations for implementation and future resaerch and finally (vi) Providing a conscluding summary

My two questions are: 1. On step 1 and 2 can it be the overall or you restate and sumamrise on each findings based on the reaerch question? 2. On 4 and 5 do you do the acknowlledgement , recommendations on each research finding or overall. This is not clear from your expalanattion.

Please respond.

This post is very useful. I’m wondering whether practical implications must be introduced in the Discussion section or in the Conclusion section?

This is very instructive and educative

Sigh, I never knew a 20 min video could have literally save my life like this. I found this at the right time!!!! Everything I need to know in one video thanks a mil ! OMGG and that 6 step!!!!!! was the cherry on top the cake!!!!!!!!!

Thanks alot.., I have gained much

This piece is very helpful on how to go about my discussion section. I can always recommend GradCoach research guides for colleagues.

Many thanks for this resource. It has been very helpful to me. I was finding it hard to even write the first sentence. Much appreciated.

Thanks so much. Very helpful to know what is included in the discussion section

this was a very helpful and useful information

This is very helpful. Very very helpful. Thanks for sharing this online!

it is very helpfull article, and i will recommend it to my fellow students. Thank you.

Superlative! More grease to your elbows.

Powerful, thank you for sharing.

Wow! Just wow! God bless the day I stumbled upon you guys’ YouTube videos! It’s been truly life changing and anxiety about my report that is due in less than a month has subsided significantly!

Simplified explanation. Well done.

The presentation is enlightening. Thank you very much.

Thanks for the support and guidance

This has been a great help to me and thank you do much

I second that “it is highly unlikely that a dissertation or thesis will scientifically prove something”; although, could you enlighten us on that comment and elaborate more please?

Sure, no problem.

Scientific proof is generally considered a very strong assertion that something is definitively and universally true. In most scientific disciplines, especially within the realms of natural and social sciences, absolute proof is very rare. Instead, researchers aim to provide evidence that supports or rejects hypotheses. This evidence increases or decreases the likelihood that a particular theory is correct, but it rarely proves something in the absolute sense.

Dissertations and theses, as substantial as they are, typically focus on exploring a specific question or problem within a larger field of study. They contribute to a broader conversation and body of knowledge. The aim is often to provide detailed insight, extend understanding, and suggest directions for further research rather than to offer definitive proof. These academic works are part of a cumulative process of knowledge building where each piece of research connects with others to gradually enhance our understanding of complex phenomena.

Furthermore, the rigorous nature of scientific inquiry involves continuous testing, validation, and potential refutation of ideas. What might be considered a “proof” at one point can later be challenged by new evidence or alternative interpretations. Therefore, the language of “proof” is cautiously used in academic circles to maintain scientific integrity and humility.

This was very helpful, thank you!

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Organizing Academic Research Papers: 8. The Discussion

• Purpose of Guide
• Design Flaws to Avoid
• Glossary of Research Terms
• Narrowing a Topic Idea
• Broadening a Topic Idea
• Extending the Timeliness of a Topic Idea
• Academic Writing Style
• Choosing a Title
• Making an Outline
• Paragraph Development
• Executive Summary
• Background Information
• The Research Problem/Question
• Theoretical Framework
• Citation Tracking
• Content Alert Services
• Evaluating Sources
• Primary Sources
• Secondary Sources
• Tertiary Sources
• What Is Scholarly vs. Popular?
• Qualitative Methods
• Quantitative Methods
• Using Non-Textual Elements
• Limitations of the Study
• Common Grammar Mistakes
• Avoiding Plagiarism
• Footnotes or Endnotes?
• Further Readings
• Annotated Bibliography
• Dealing with Nervousness
• Using Visual Aids
• Grading Someone Else's Paper
• How to Manage Group Projects
• Multiple Book Review Essay
• Reviewing Collected Essays
• About Informed Consent
• Writing Field Notes
• Writing a Policy Memo
• Writing a Research Proposal
• Acknowledgements

The purpose of the discussion is to interpret and describe the significance of your findings in light of what was already known about the research problem being investigated, and to explain any new understanding or fresh insights about the problem after you've taken the findings into consideration. The discussion will always connect to the introduction by way of the research questions or hypotheses you posed and the literature you reviewed, but it does not simply repeat or rearrange the introduction; the discussion should always explain how your study has moved the reader's understanding of the research problem forward from where you left them at the end of the introduction.

Importance of a Good Discussion

This section is often considered the most important part of a research paper because it most effectively demonstrates your ability as a researcher to think critically about an issue, to develop creative solutions to problems based on the findings, and to formulate a deeper, more profound understanding of the research problem you are studying.

The discussion section is where you explore the underlying meaning of your research , its possible implications in other areas of study, and the possible improvements that can be made in order to further develop the concerns of your research.

This is the section where you need to present the importance of your study and how it may be able to contribute to and/or fill existing gaps in the field. If appropriate, the discussion section is also where you state how the findings from your study revealed new gaps in the literature that had not been previously exposed or adequately described.

This part of the paper is not strictly governed by objective reporting of information but, rather, it is where you can engage in creative thinking about issues through evidence-based interpretation of findings. This is where you infuse your results with meaning.

Kretchmer, Paul. Fourteen Steps to Writing to Writing an Effective Discussion Section . San Francisco Edit, 2003-2008.

Structure and Writing Style

I.  General Rules

These are the general rules you should adopt when composing your discussion of the results :

• Do not be verbose or repetitive.
• Be concise and make your points clearly.
• Avoid using jargon.
• Follow a logical stream of thought.
• Use the present verb tense, especially for established facts; however, refer to specific works and references in the past tense.
• If needed, use subheadings to help organize your presentation or to group your interpretations into themes.

II.  The Content

The content of the discussion section of your paper most often includes :

• Explanation of results : comment on whether or not the results were expected and present explanations for the results; go into greater depth when explaining findings that were unexpected or especially profound. If appropriate, note any unusual or unanticipated patterns or trends that emerged from your results and explain their meaning.
• References to previous research : compare your results with the findings from other studies, or use the studies to support a claim. This can include re-visiting key sources already cited in your literature review section, or, save them to cite later in the discussion section if they are more important to compare with your results than being part of the general research you cited to provide context and background information.
• Deduction : a claim for how the results can be applied more generally. For example, describing lessons learned, proposing recommendations that can help improve a situation, or recommending best practices.
• Hypothesis : a more general claim or possible conclusion arising from the results [which may be proved or disproved in subsequent research].

III. Organization and Structure

Keep the following sequential points in mind as you organize and write the discussion section of your paper:

• Think of your discussion as an inverted pyramid. Organize the discussion from the general to the specific, linking your findings to the literature, then to theory, then to practice [if appropriate].
• Use the same key terms, mode of narration, and verb tense [present] that you used when when describing the research problem in the introduction.
• Begin by briefly re-stating the research problem you were investigating and answer all of the research questions underpinning the problem that you posed in the introduction.
• Describe the patterns, principles, and relationships shown by each major findings and place them in proper perspective. The sequencing of providing this information is important; first state the answer, then the relevant results, then cite the work of others. If appropriate, refer the reader to a figure or table to help enhance the interpretation of the data. The order of interpreting each major finding should be in the same order as they were described in your results section.
• A good discussion section includes analysis of any unexpected findings. This paragraph should begin with a description of the unexpected finding, followed by a brief interpretation as to why you believe it appeared and, if necessary, its possible significance in relation to the overall study. If more than one unexpected finding emerged during the study, describe each them in the order they appeared as you gathered the data.
• Before concluding the discussion, identify potential limitations and weaknesses. Comment on their relative importance in relation to your overall interpretation of the results and, if necessary, note how they may affect the validity of the findings. Avoid using an apologetic tone; however, be honest and self-critical.
• The discussion section should end with a concise summary of the principal implications of the findings regardless of statistical significance. Give a brief explanation about why you believe the findings and conclusions of your study are important and how they support broader knowledge or understanding of the research problem. This can be followed by any recommendations for further research. However, do not offer recommendations which could have been easily addressed within the study. This demonstrates to the reader you have inadequately examined and interpreted the data.

IV.  Overall Objectives

The objectives of your discussion section should include the following: I.  Reiterate the Research Problem/State the Major Findings

Briefly reiterate for your readers the research problem or problems you are investigating and the methods you used to investigate them, then move quickly to describe the major findings of the study. You should write a direct, declarative, and succinct proclamation of the study results.

II.  Explain the Meaning of the Findings and Why They are Important

No one has thought as long and hard about your study as you have. Systematically explain the meaning of the findings and why you believe they are important. After reading the discussion section, you want the reader to think about the results [“why hadn’t I thought of that?”]. You don’t want to force the reader to go through the paper multiple times to figure out what it all means. Begin this part of the section by repeating what you consider to be your most important finding first.

III.  Relate the Findings to Similar Studies

No study is so novel or possesses such a restricted focus that it has absolutely no relation to other previously published research. The discussion section should relate your study findings to those of other studies, particularly if questions raised by previous studies served as the motivation for your study, the findings of other studies support your findings [which strengthens the importance of your study results], and/or they point out how your study differs from other similar studies. IV.  Consider Alternative Explanations of the Findings

It is important to remember that the purpose of research is to discover and not to prove . When writing the discussion section, you should carefully consider all possible explanations for the study results, rather than just those that fit your prior assumptions or biases.

V.  Acknowledge the Study’s Limitations

It is far better for you to identify and acknowledge your study’s limitations than to have them pointed out by your professor! Describe the generalizability of your results to other situations, if applicable to the method chosen, then describe in detail problems you encountered in the method(s) you used to gather information. Note any unanswered questions or issues your study did not address, and.... VI.  Make Suggestions for Further Research

Although your study may offer important insights about the research problem, other questions related to the problem likely remain unanswered. Moreover, some unanswered questions may have become more focused because of your study. You should make suggestions for further research in the discussion section.

NOTE: Besides the literature review section, the preponderance of references to sources in your research paper are usually found in the discussion section . A few historical references may be helpful for perspective but most of the references should be relatively recent and included to aid in the interpretation of your results and/or linked to similar studies. If a study that you cited disagrees with your findings, don't ignore it--clearly explain why the study's findings differ from yours.

V.  Problems to Avoid

• Do not waste entire sentences restating your results . Should you need to remind the reader of the finding to be discussed, use "bridge sentences" that relate the result to the interpretation. An example would be: “The lack of available housing to single women with children in rural areas of Texas suggests that...[then move to the interpretation of this finding].”
• Recommendations for further research can be included in either the discussion or conclusion of your paper but do not repeat your recommendations in the both sections.
• Do not introduce new results in the discussion. Be wary of mistaking the reiteration of a specific finding for an interpretation.
• Use of the first person is acceptable, but too much use of the first person may actually distract the reader from the main points.

Analyzing vs. Summarizing. Department of English Writing Guide. George Mason University; Discussion . The Structure, Format, Content, and Style of a Journal-Style Scientific Paper. Department of Biology. Bates College; Hess, Dean R. How to Write an Effective Discussion. Respiratory Care 49 (October 2004); Kretchmer, Paul. Fourteen Steps to Writing to Writing an Effective Discussion Section . San Francisco Edit, 2003-2008; The Lab Report . University College Writing Centre. University of Toronto; Summary: Using it Wisely . The Writing Center. University of North Carolina; Schafer, Mickey S. Writing the Discussion . Writing in Psychology course syllabus. University of Florida; Yellin, Linda L. A Sociology Writer's Guide. Boston, MA: Allyn and Bacon, 2009.

Writing Tip

Don’t Overinterpret the Results!

Interpretation is a subjective exercise. Therefore, be careful that you do not read more into the findings than can be supported by the evidence you've gathered. Remember that the data are the data: nothing more, nothing less.

Another Writing Tip

Don't Write Two Results Sections!

One of the most common mistakes that you can make when discussing the results of your study is to present a superficial interpretation of the findings that more or less re-states the results section of your paper. Obviously, you must refer to your results when discussing them, but focus on the interpretion of those results, not just the data itself.

Azar, Beth. Discussing Your Findings.  American Psychological Association gradPSYCH Magazine (January 2006)

Yet Another Writing Tip

Avoid Unwarranted Speculation!

The discussion section should remain focused on the findings of your study. For example, if you studied the impact of foreign aid on increasing levels of education among the poor in Bangladesh, it's generally not appropriate to speculate about how your findings might apply to populations in other countries without drawing from existing studies to support your claim. If you feel compelled to speculate, be certain that you clearly identify your comments as speculation or as a suggestion for where further research is needed. Sometimes your professor will encourage you to expand the discussion in this way, while others don’t care what your opinion is beyond your efforts to interpret the data.

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Writing a scientific paper.

• Writing a lab report
• INTRODUCTION

Writing a "good" discussion section

"discussion and conclusions checklist" from: how to write a good scientific paper. chris a. mack. spie. 2018., peer review.

• LITERATURE CITED
• Bibliography of guides to scientific writing and presenting
• Presentations
• Lab Report Writing Guides on the Web

This is is usually the hardest section to write. You are trying to bring out the true meaning of your data without being too long. Do not use words to conceal your facts or reasoning. Also do not repeat your results, this is a discussion.

• Present principles, relationships and generalizations shown by the results
• Point out exceptions or lack of correlations. Define why you think this is so.
• Show how your results agree or disagree with previously published works
• Discuss the theoretical implications of your work as well as practical applications
• State your conclusions clearly. Summarize your evidence for each conclusion.
• Discuss the significance of the results
•  Evidence does not explain itself; the results must be presented and then explained.
• Typical stages in the discussion: summarizing the results, discussing whether results are expected or unexpected, comparing these results to previous work, interpreting and explaining the results (often by comparison to a theory or model), and hypothesizing about their generality.
• Discuss any problems or shortcomings encountered during the course of the work.
• Discuss possible alternate explanations for the results.
• Avoid: presenting results that are never discussed; presenting discussion that does not relate to any of the results; presenting results and discussion in chronological order rather than logical order; ignoring results that do not support the conclusions; drawing conclusions from results without logical arguments to back them up. 

CONCLUSIONS

• Provide a very brief summary of the Results and Discussion.
• Emphasize the implications of the findings, explaining how the work is significant and providing the key message(s) the author wishes to convey.
• Provide the most general claims that can be supported by the evidence.
• Provide a future perspective on the work.
• Avoid: repeating the abstract; repeating background information from the Introduction; introducing new evidence or new arguments not found in the Results and Discussion; repeating the arguments made in the Results and Discussion; failing to address all of the research questions set out in the Introduction. 

WHAT HAPPENS AFTER I COMPLETE MY PAPER?

 The peer review process is the quality control step in the publication of ideas.  Papers that are submitted to a journal for publication are sent out to several scientists (peers) who look carefully at the paper to see if it is "good science".  These reviewers then recommend to the editor of a journal whether or not a paper should be published. Most journals have publication guidelines. Ask for them and follow them exactly.    Peer reviewers examine the soundness of the materials and methods section.  Are the materials and methods used written clearly enough for another scientist to reproduce the experiment?  Other areas they look at are: originality of research, significance of research question studied, soundness of the discussion and interpretation, correct spelling and use of technical terms, and length of the article.

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Ever find yourself stuck when trying to write the discussion part of your research paper? Don't worry, it happens to a lot of people. 

The discussion section is super important in your research paper . It's where you explain what your results mean. But turning all that data into a clear and meaningful story? That's not easy.

Guess what? MyPerfectWords.com has come up with a solution. 

This blog is your guide to writing an outstanding discussion section. We'll guide you step by step with useful tips to make sure your research stands out.

So, let’s get started!

• 1. What Exactly is a Discussion Section in the Research Paper?
• 2. How to Write the Discussion Section of a Research Paper?
• 3. Examples of Good Discussion for a Research Paper
• 4. Mistakes to Avoid in Your Research Paper's Discussion 

What Exactly is a Discussion Section in the Research Paper?

In a research paper, the discussion section is where you explain what your results really mean. It's like answering the questions, "So what?" and "What's the big picture?" 

The discussion section is your chance to help your readers understand why your findings are important and how they fit into the larger context. It's more than just summarizing; it's about making your research understandable and meaningful to others.

Importance of the Discussion Section

The discussion section isn't just a formality; it's the heart of your research paper. This is where your findings transform from data into knowledge. 

Let's break down why it's so crucial:

• Interpretation of Results : The discussion is where you get to tell readers what your results really mean. You go into the details, helping them understand the story behind the numbers or findings.
• Connecting the Dots : You connect different parts of your research, showing how they relate. This helps your readers see the bigger picture.
• Relevance to the Big Picture : You get to highlight why your research matters. How does it contribute to the broader understanding of the topic? This is your time to make your research significant.
• Addressing Limitations : In the discussion, you can acknowledge any limitations in your study and discuss how they might impact your results.
• Suggestions for Further Research : The discussion is where you suggest areas for future exploration. It's like passing the baton to the next researcher, indicating where more work could be done.

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How to Write the Discussion Section of a Research Paper?

The Discussion section in a research paper plays a vital role in interpreting findings and formulating a conclusion . Given below are the main components of the discussion section:

• Quick Summary: A brief recap of your main findings.
• Interpretation: Significance and meaning of your results in relation to your research question.
• Literature Review : Connecting your findings with previous research or similar studies.
• Limitations: Discussing any study limitations, addressing potential concerns.
• Implications: Broader implications of your findings, considering practical and theoretical aspects.
• Alternative Explanations: Evaluating alternative interpretations, demonstrating a comprehensive analysis.
• Connecting to Hypotheses : Summarizing how your result section aligns or diverges from your initial hypotheses.

Now let’s explore the steps to write an effective discussion section that will effectively communicate the significance of your research:

Step 1: Get Started with a Quick Summary

Start by quickly telling your readers the main things you found in your research. Don't explain them in detail just yet; just give a simple overview. 

This helps your readers get the big picture before diving into the details.

Step 2: Interpret Your Results

In the next step, talk about what your findings really mean. Share why the information you gathered is important. Connect each result to the questions you were trying to answer and the goals you set for your research.

Step 3: Relate to Existing Literature

In this step, link up your discoveries with what other researchers have already figured out. 

Share if your results are similar to or different from what's been found before. This helps give more background to your study and shows you know what other scientists have been up to.

Step 4: Address Limitations Honestly

Every study has its limitations. Acknowledge them openly in your discussion. This not only shows transparency but also helps readers interpret your results more accurately.

Step 5: Discuss the Implications

Explore the implications of your findings. How do they contribute to the field? What real-world applications or changes might they suggest?

Dig into why your discoveries are important. How do they help the subject you studied? 

This step is like looking at the bigger picture and asking, "So, what can we do with this information?"

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Step 6: Consider Alternative Explanations

After discussing the implications, challenge yourself by exploring alternative explanations for your results. 

Discuss different perspectives and show that you've considered multiple angles.

Step 7: Connect to Your Hypotheses or Research Questions

For the last step, revisit your initial hypotheses or research questions. Explain whether your results support what you thought might happen or if they surprised you. 

Examples of Good Discussion for a Research Paper

Learning from well-crafted discussions can significantly enhance your own writing. Given below are some examples to help you understand how to write your own.

Discussion for a Research Paper Example Pdf

Discussion for a Medical Research Paper

Discussion Section for a Qualitative Research Paper

Mistakes to Avoid in Your Research Paper's Discussion 

Writing the discussion section of your research paper can be tricky. To make sure you're on the right track, be mindful of these common mistakes:

• Overstating or Overinterpreting Results

Avoid making your findings sound more groundbreaking than they are. Stick to what your data actually shows, and don't exaggerate.

• Neglecting Alternative Explanations 

Failing to consider other possible explanations for your results can weaken your discussion. Always explore alternative perspectives to present a well-rounded view.

• Ignoring Limitations 

Don't sweep limitations under the rug. Acknowledge them openly and discuss how they might affect the validity or generalizability of your results.

• Being Overly Technical or Jargon-laden

Remember that your audience may not be experts in your specific field. Avoid using overly technical language or excessive jargon that could alienate your readers.

• Disregarding the 'So What' Factor

Always explain the significance of your findings. Don't leave your readers wondering why your research matters or how it contributes to the broader understanding of the subject.

• Rushing the Conclusion

The conclusion section of your discussion is critical. Don't rush it. Summarize the key points and leave your readers with a strong understanding of the significance of your research.

So, there you have it —writing a discussion and conclusion section isn't easy, but avoiding some common mistakes can make it much smoother. 

Remember to keep it real with your results, think about what else could explain things, and don't forget about any limits in your study.

But if you're feeling stuck, MyPerfectWords.com is here for you. 

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Writing a Discussion Section

Writing a discussion section is where you really begin to add your interpretations to the work.

This article is a part of the guide:

• Outline Examples
• Example of a Paper
• Write a Hypothesis
• Introduction

Browse Full Outline

• 1 Write a Research Paper
• 2 Writing a Paper
• 3.1 Write an Outline
• 3.2 Outline Examples
• 4.1 Thesis Statement
• 4.2 Write a Hypothesis
• 5.2 Abstract
• 5.3 Introduction
• 5.4 Methods
• 5.5 Results
• 5.6 Discussion
• 5.7 Conclusion
• 5.8 Bibliography
• 6.1 Table of Contents
• 6.2 Acknowledgements
• 6.3 Appendix
• 7.1 In Text Citations
• 7.2 Footnotes
• 7.3.1 Floating Blocks
• 7.4 Example of a Paper
• 7.5 Example of a Paper 2
• 7.6.1 Citations
• 7.7.1 Writing Style
• 7.7.2 Citations
• 8.1.1 Sham Peer Review
• 8.1.2 Advantages
• 8.1.3 Disadvantages
• 8.2 Publication Bias
• 8.3.1 Journal Rejection
• 9.1 Article Writing
• 9.2 Ideas for Topics

In this critical part of the research paper, you start the process of explaining any links and correlations apparent in your data.

If you left few interesting leads and open questions in the results section , the discussion is simply a matter of building upon those and expanding them.

The Difficulties of Writing a Discussion Section

In an ideal world, you could simply reject your null or alternative hypotheses according to the significance levels found by the statistics.

That is the main point of your discussion section, but the process is usually a lot more complex than that. It is rarely clear-cut, and you will need to interpret your findings.

For example, one of your graphs may show a distinct trend, but not enough to reach an acceptable significance level.

Remember that no significance is not the same as no difference, and you can begin to explain this in your discussion section.

Whilst your results may not be enough to reject the null hypothesis , they may show a trend that later researchers may wish to explore, perhaps by refining the experiment .

Self-Criticism at the Heart of Writing a Discussion Section

For this purpose, you should criticize the experiment, and be honest about whether your design was good enough. If not, suggest any modifications and improvements that could be made to the design.

Maybe the reason that you did not find a significant correlation is because your sampling was not random , or you did not use sensitive enough equipment.

The discussion section is not always about what you found, but what you did not find, and how you deal with that. Stating that the results are inconclusive is the easy way out, and you must always try to pick out something of value.

Using the Discussion Section to Expand Knowledge

You should always put your findings into the context of the previous research that you found during your literature review . Do your results agree or disagree with previous research?

Do the results of the previous research help you to interpret your own findings? If your results are very different, why? Either you have uncovered something new, or you may have made a major flaw with the design of the experiment .

Finally, after saying all of this, you can make a statement about whether the experiment has contributed to knowledge in the field, or not.

Unless you made so many errors that the results are completely unreliable, you will; certainly have learned something. Try not to be too broad in your generalizations to the wider world - it is a small experiment and is unlikely to change the world.

Once writing the discussion section is complete, you can move onto the next stage, wrapping up the paper with a focused conclusion .

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• How to Write a Discussion Section | Tips & Examples

How to Write a Discussion Section | Tips & Examples

Published on 21 August 2022 by Shona McCombes . Revised on 25 October 2022.

The discussion section is where you delve into the meaning, importance, and relevance of your results .

It should focus on explaining and evaluating what you found, showing how it relates to your literature review , and making an argument in support of your overall conclusion . It should not be a second results section .

There are different ways to write this section, but you can focus your writing around these key elements:

• Summary: A brief recap of your key results
• Interpretations: What do your results mean?
• Implications: Why do your results matter?
• Limitations: What can’t your results tell us?
• Recommendations: Avenues for further studies or analyses

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Table of contents

What not to include in your discussion section, step 1: summarise your key findings, step 2: give your interpretations, step 3: discuss the implications, step 4: acknowledge the limitations, step 5: share your recommendations, discussion section example.

There are a few common mistakes to avoid when writing the discussion section of your paper.

• Don’t introduce new results: You should only discuss the data that you have already reported in your results section .
• Don’t make inflated claims: Avoid overinterpretation and speculation that isn’t directly supported by your data.
• Don’t undermine your research: The discussion of limitations should aim to strengthen your credibility, not emphasise weaknesses or failures.

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Start this section by reiterating your research problem  and concisely summarising your major findings. Don’t just repeat all the data you have already reported – aim for a clear statement of the overall result that directly answers your main  research question . This should be no more than one paragraph.

Many students struggle with the differences between a discussion section and a results section . The crux of the matter is that your results sections should present your results, and your discussion section should subjectively evaluate them. Try not to blend elements of these two sections, in order to keep your paper sharp.

• The results indicate that …
• The study demonstrates a correlation between …
• This analysis supports the theory that …
• The data suggest  that …

The meaning of your results may seem obvious to you, but it’s important to spell out their significance for your reader, showing exactly how they answer your research question.

The form of your interpretations will depend on the type of research, but some typical approaches to interpreting the data include:

• Identifying correlations , patterns, and relationships among the data
• Discussing whether the results met your expectations or supported your hypotheses
• Contextualising your findings within previous research and theory
• Explaining unexpected results and evaluating their significance
• Considering possible alternative explanations and making an argument for your position

You can organise your discussion around key themes, hypotheses, or research questions, following the same structure as your results section. Alternatively, you can also begin by highlighting the most significant or unexpected results.

• In line with the hypothesis …
• Contrary to the hypothesised association …
• The results contradict the claims of Smith (2007) that …
• The results might suggest that x . However, based on the findings of similar studies, a more plausible explanation is x .

As well as giving your own interpretations, make sure to relate your results back to the scholarly work that you surveyed in the literature review . The discussion should show how your findings fit with existing knowledge, what new insights they contribute, and what consequences they have for theory or practice.

Ask yourself these questions:

• Do your results support or challenge existing theories? If they support existing theories, what new information do they contribute? If they challenge existing theories, why do you think that is?
• Are there any practical implications?

Your overall aim is to show the reader exactly what your research has contributed, and why they should care.

• These results build on existing evidence of …
• The results do not fit with the theory that …
• The experiment provides a new insight into the relationship between …
• These results should be taken into account when considering how to …
• The data contribute a clearer understanding of …
• While previous research has focused on  x , these results demonstrate that y .

Even the best research has its limitations. Acknowledging these is important to demonstrate your credibility. Limitations aren’t about listing your errors, but about providing an accurate picture of what can and cannot be concluded from your study.

Limitations might be due to your overall research design, specific methodological choices , or unanticipated obstacles that emerged during your research process.

Here are a few common possibilities:

• If your sample size was small or limited to a specific group of people, explain how generalisability is limited.
• If you encountered problems when gathering or analysing data, explain how these influenced the results.
• If there are potential confounding variables that you were unable to control, acknowledge the effect these may have had.

After noting the limitations, you can reiterate why the results are nonetheless valid for the purpose of answering your research question.

• The generalisability of the results is limited by …
• The reliability of these data is impacted by …
• Due to the lack of data on x , the results cannot confirm …
• The methodological choices were constrained by …
• It is beyond the scope of this study to …

Based on the discussion of your results, you can make recommendations for practical implementation or further research. Sometimes, the recommendations are saved for the conclusion .

Suggestions for further research can lead directly from the limitations. Don’t just state that more studies should be done – give concrete ideas for how future work can build on areas that your own research was unable to address.

• Further research is needed to establish …
• Future studies should take into account …
• Avenues for future research include …

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Journal Article: Discussion

Criteria for success.

A strong Discussion section:

• Tells the main conclusion of the paper in one or two sentences.
• Tells how the paper’s results contribute to answering the big questions posed in the Introduction.
• Explains how (and why) this work agrees or disagrees with other, similar work.
• Explains how the limitations of this study leave the big questions unanswered.
• Tells how extensions of this paper’s results will be useful for answering the big questions.

Structure Diagram

The Discussion is the part of your paper where you can share what you think your results mean with respect to the big questions you posed in your Introduction. The Introduction and Discussion are natural partners: the Introduction tells the reader what question you are working on and why you did this experiment to investigate it; the Discussion tells the reader what the results of that experiment have to say about the bigger question.

Imagine you explained the results in the paper to a labmate who looks confused and asks you, “Sure, but so what? Why was this cool or interesting?” Your response to your labmate should be similar to the content in the Discussion.

Analyze Your Audience

Different kinds of readers will expect different things from your Discussion. Readers who are not experts in your field might read your Discussion before your Results in the hopes that they can learn what your Results mean and why your paper is important without having to learn how to interpret your experimental results. They might also be interested to know what you think the future of your field is. Readers who are more familiar with your field will generally understand what the results of your experiments say, but they will be curious about how you interpreted confusing, conflicting, or complicated results.

As you write your Discussion, decide who will find each paragraph interesting and what you want them to take away from it. Successful Discussions can simultaneously provide the specific, nuanced information that experts want to read and the broader, more general statements that non-experts can appreciate.

The balance between expert and non-expert readers in your target audience will depend on the journal to which you submit. High-profile, general readership journals will have more non-expert readers, while more technical, field-specific journals have almost exclusively expert readers.

Tell how your paper is special

Weak Discussions begin with a summary of the results or a repetition of the main points of the Introduction. Strong Discussions immediately carve out a place for themselves in the large universe of papers by saying what makes this one interesting or special. One way to do this is to start the Discussion with one or two sentences that state the main finding from the results and what that finding means for the field.

Relate your results to existing results

In the Introduction, you probably helped motivate your study by citing previous results in your field. Now that you’ve laid out your results, you should tell whether your results agree or disagree with prior work and why. You might have extended previous work, showed how apparently conflicting results are actually harmonious, or exposed a contradiction that currently has no explanation.

Tell how your study’s limitations leave open the big questions

Every study is finite: you did some things and not others, and you used methods that can explain some phenomena but not others. How do the limitations of your study leave open the bigger questions? Do you just need to do more of the same kind of work? Have you shown that current methods are inadequate for answering the big question?

Every paper is a contribution to a larger scientific conversation. Hopefully, you think your contribution is somehow useful to that conversation: it provides new information or tools that will help you or other researchers move toward answers to the big questions. To explain this contribution, many Discussions end with a forward-looking statement that tries to place the paper in an expected future of research in that field.

This content was adapted from from an article originally created by the  MIT Biological Engineering Communication Lab .

Resources and Annotated Examples

Annotated example 1.

This is the discussion for an article published in Science Translational Medicine . 6 MB

Annotated Example 2

This is the discussion for an article published in Cell . 325 KB

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Fangtao Zhu

Cunwei cheng, associated data.

Varicose veins of the lower extremities (VVs) is a common chronic vascular disease, with high prevalence rates in some countries; however, their pathogenesis remains unclear. Some studies have identified associations between changes in specific plasma lipid molecules, such as phosphatidylethanolamine (PE), phosphatidylcholine (PC), and sphingomyelin (SM), and the onset of VVs, but due to confounders and reverse causality, the causal relationship remains unclear. Meanwhile, studies on the potential link between other plasma lipids beyond PE, PC, and SM and the risk of VVs in the lower extremities are lacking. This study aimed to explore the potential causal relationship between VVs and plasma lipid levels to provide theoretical insights into the interrelation of plasma lipids and VVs in their occurrence and progression. We conducted a two-sample Mendelian randomization (MR) analysis to assess the potential connection between genetically predicted levels of individual plasma lipids and the risk of developing VVs. We utilized data from a large-scale genome-wide association study involving 7174 Finnish individuals for 179 plasma lipidomes along with VVs genome-wide association study data from 408,455 UK individuals. MR analysis employed methods, such as inverse-variance weighting, weighted median, Bayesian Weighted Mendelian Randomization, and MR-Egger regression. The inverse-variance weighting method was primarily used to assess causality. The validity of the results was demonstrated through sensitivity analysis. In total, 12 lipids were found to have their plasma levels associated with an increased risk of VVs. This includes 3 types of PE, 7 types of PC, and 2 types of phosphatidylinositol. However, no significant causal relationship was found between the plasma levels of 11 types of SM and VVs. These results support the existence of a potential causal relationship between specific types of lipid levels and the risk of VVs, which can provide clues for further studies on biological mechanisms and the exploration of potential therapeutic targets.

1. Introduction

Varicose veins are a common vascular disease, with an incidence rate of 50% to 60% in some Western countries, making it one of the most common chronic diseases in Western medicine, with varicose veins of the lower extremities (VVs) being the most prevalent type. [ 1 , 2 ] The exact pathogenesis of VVs is complex, involving various risk factors, such as genetics, age, sex, metabolism, and lifestyle. [ 3 ] To date, the morphological characteristics of the disease have been well-documented, with known histological changes in dilated veins, including intimal hyperplasia and the destruction of smooth muscle cells and extracellular matrix. [ 4 ] Other contributing factors in its development include changes in hemodynamics, such as reduced laminar shear stress and increased venous filling pressure, endothelial activation, inflammation, hypoxia, and the dysregulation of matrix metalloproteinases and their tissue inhibitors. [ 5 – 8 ] Additionally, in literature, the familial aggregation and heritability of the condition have been widely recognized. [ 9 – 12 ] However, how disease variants at risk loci disrupt cellular biochemistry and how these events translate into the formation of varicose veins remain unclear, thus necessitating research to determine how biochemical abnormalities in VVs regulate metabolic pathways. The treatment of VVs primarily focuses on conservative and surgical methods, while the effectiveness of pharmacological treatment and early prevention is limited. [ 13 – 15 ] Therefore, exploring the pathogenesis of VVs can provide more insights into the prevention and treatment of this disease. Abnormal levels of certain lipid molecules, such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), and sphingomyelin (SM), in varicose veins, indicate that these lipids might play a significant role in the progression of the condition, particularly in aspects related to inflammation and cellular pathways. [ 6 , 16 ] However, the causal impact of lipids on VVs remains unclear, and the relationship between other plasma lipid components and VVs has not been studied.

Mendelian randomization (MR) is a method used in epidemiology to assess the potential causal relationships between specific risk factors and diseases. It mitigates issues of confounders and reverse causality by using genetic variations as tools for a natural experiment. This method is similar to traditional randomized controlled trials, in that it simulates experimental conditions through the random allocation of genes, thus reducing study biases. The MR method can mitigate issues of confounding, reverse causality, and the challenges of representativeness and feasibility inherent in observational studies and randomized controlled trials, respectively. [ 17 ] However, no study has explored the relationship between plasma lipid levels and VVs using the MR method.

Therefore, this study aimed to explore the potential causal relationship between VVs and plasma lipid levels to provide theoretical insights into the interrelation of plasma lipids and VVs in their occurrence and progression using the two-sample MR approach.

2.1. Research design

MR methods must satisfy 3 core assumptions: association, independence, and exclusion-restriction (Fig. ​ (Fig.1). 1 ). Specifically: ① the instrumental variable must be strongly associated with the exposure factor; ② the instrumental variable should not be associated with any confounders related to the “exposure–outcome” relationship; ③ The instrumental variable can only influence the outcome variable through the exposure factor.

The 3 key assumptions of MR research are: (1) SNP is closely related to various lipid levels; (2) SNP is independent of other known confounding factors; (3) SNP only affects the risk of VVs through various lipids. X indicates that the SNP chosen as an instrumental variable is not directly related to confounding factors and outcomes.

In this study, we conducted independent two-sample MR analyses on 179 types of plasma lipids, utilizing summary data from genome-wide association studies (GWAS) to evaluate their causal relationship with VVs and to screen for lipid types with positive results, as well as sensitivity analyses to test the reliability of the results. The study workflow is illustrated in Figure ​ Figure2 2 .

The flowchart of the study.

2.2. Data sources

The analysis utilized publicly available summary statistics from published GWAS, primarily focusing on European populations, including both males and females. For lipids GWAS data, we used a study whose univariate GWAS summary statistics are stored in the GWAS Catalog with accession numbers GCST90277238 to GCST90277416. This study includes GWAS data on 179 lipid species across 13 lipid categories (phosphatidylinositol [PI], phosphatidylethanolamine-ether [PEO], PE, phosphatidylcholine-ether [PCO], PC, Lyso-phosphatidylethanolamine [LPE], Lyso-phosphatidylcholine [LPC], Triacylglycerol [TAG], Diacylglycerol [DAG], Ceramide [Cer], SM, Cholesteryl ester [CE], Cholesterol [Chol]), covering 4 major lipid categories (glycerolipids, glycerophospholipids, sphingolipids, and sterols) from 7174 Finnish individuals. This study’s lipid subdivision is based on their chemical structural differences, including the length of fatty acid chains, their saturation level (i.e., the number of double bonds), and their position on the molecular backbone. These subtle chemical differences are crucial for the biological functions of lipids. For instance, PC (15:0_18:1) contains a 15-carbon saturated fatty acid chain at the sn-1 position and an 18-carbon fatty acid chain with 1 double bond at the sn-2 position. Again for instance, in PC (O-17:0_17:1), “O-17:0” indicates that the fatty acid at the sn-1 position is a 17-carbon saturated fatty acid linked via an ether bond, while “17:1” indicates that the fatty acid at the sn-2 position is a 17-carbon unsaturated fatty acid with 1 double bond, connected via a conventional ester linkage. Ether-linked phospholipids exhibit unique biological properties, such as increased resistance to oxidation and thermal stability, which may affect the fluidity of cell membranes and cellular functions. These structural differences affect the fluidity, curvature, and signaling functions of cell membranes, potentially influencing the pathophysiology of various diseases. [ 18 ] The GWAS summary statistics for lower extremity varicose veins were derived from a pan-ancestry genetic analysis by Gene ATLAS, including 10,861 cases and 397,594 controls of British ancestry, all cases and controls in this study were from the UK Biobank. [ 19 ] The UK Biobank is a prospective study that recruited over half a million participants aged 40 to 69 years between 2006 and 2010. It collected extensive phenotypic and genotypic details of the participants, including whole-genome genotyping and a wide range of health-related data. [ 20 ] Since the GWAS data used in this study are publicly accessible ( https://www.ebi.ac.uk/gwas/home ) and have received ethical clearance from relevant review boards, our analysis did not necessitate further ethical approval. The summary information for the significant exposure factors is displayed in Table ​ Table1 1 .

The summary information for the significant exposure factors in this study.

PC = phosphatidylcholine, PE = phosphatidylethanolamine, PI = phosphatidylinositol, VVs = varicose veins of lower extremity.

2.3. Selection and validation of instrumental variable SNPs

We selected single nucleotide polymorphisms (SNPs) associated with 179 plasma lipid levels using 3 criteria. First, we chose SNPs that reached genome-wide significance levels ( P  < 5 × 10 −5 ). Second, we assessed the independence of these selected SNPs through linkage disequilibrium analysis. SNPs in linkage disequilibrium and close to other SNPs with higher P -values were excluded; specifically, we removed SNPs within a 10,000 kb range of the most significant SNP with r 2  > 0.001. Finally, to control for potential bias, our selected instrumental variables were evaluated for weak instrument bias through the calculation of F-statistics. An F-statistic >10 indicates the absence of weak instrument bias, thereby further validating the association assumption. The formula for calculating the F-statistic is F = [(N − K − 1)/ K] × [ R ²/ (1 −  R ²)], where N is the sample size for the exposure factor, K is the number of instrumental variables, and R ² is the proportion of variance in the exposure factor explained by the instrumental variables. The information about the SNPs that are statistically significant with VVs can be found in Table ​ Table2 2 and Table S1 to S12, Supplemental Digital Content, http://links.lww.com/MD/N476 . Table S1 to S12, Supplemental Digital Content, http://links.lww.com/MD/N476 which illustrate SNPs of each lipids with statistically significant threshold.

Associations between the genetically predicted plasma levels of lipids and the risk of VVs.

PC = phosphatidylcholine, PE = phosphatidylethanolamine, PI = phosphatidylinositol.

2 .4. Two-sample MR analysis

Two-sample Mendelian randomization analysis was conducted using random-effects inverse-variance weighted (IVW), weighted median, and MR-Egger methods to assess the potential causal relationship between plasma lipids and VVs. Traditional IVW analysis may be susceptible to bias from invalid instruments or pleiotropy. Therefore, this study incorporates sensitivity analyses to test the validity and robustness of the IVW results. Additionally, we incorporated Bayesian Weighted Mendelian Randomization (BWMR) into our analysis. BWMR can account for uncertainties in the estimated weak effects and weak horizontal pleiotropy effects and adaptively detect outliers caused by some larger horizontal pleiotropy effects. [ 21 ] Further validation through BWMR indicates more robust and reliable results.

Sensitivity analysis is a crucial step in assessing the robustness of study results. It is designed to detect and quantify the impact of potential biases and heterogeneity, as well as the influence of individual genetic variations on the overall effect estimate. In this study, the average level of pleiotropy bias was assessed through MR-Egger regression, where P  < .05 indicates the presence of pleiotropy between the exposure and outcome variables. MR-PRESSO is utilized to detect horizontal pleiotropy. For heterogeneity testing, we employ Cochran Q method, with P  < .05 indicating the presence of heterogeneity among the included instrumental variable SNPs, suggesting that the study results are influenced by heterogeneity. The “leave-one-out” method involves sequentially removing each SNP and then conducting MR analysis with the remaining SNPs to observe changes in the overall effect estimate. If the removal of any single SNP results in a significant change in the effect estimate, this may indicate that the SNP has an undue influence on the overall estimate. The results of the sensitivity tests support the robustness of the research methodology and the reliability of the findings.

All statistical analyses were conducted using the “TwoSampleMR” package in R software, version 4.3.2.

After conducting a two-sample MR analysis, we confirmed a potential causal relationship between 12 types of lipids and the risk of VVs. This includes 7 types of PC (phosphatidylcholine [15:0_18:1], phosphatidylcholine [17:0_20:4], phosphatidylcholine [18:2_20:4], phosphatidylcholine [O-16:0_16:0], phosphatidylcholine [O-17:0_17:1], phosphatidylcholine [O-18:0_20:4], phosphatidylcholine [O-18:2_20:4]), 3 types of PE (phosphatidylethanolamine [O-16:1_20:4], phosphatidylethanolamine [O-16:1_22:5], phosphatidylethanolamine [O-18:1_20:4]), and 2 types of PI (phosphatidylinositol [18:0_18:2], phosphatidylinositol [18:1_18:2]). Among them, PC (15:0_18:1) showed an odds ratio (OR) of 1.002, 95% confidence interval (CI) of 1.001 to 1.005, and a P -value of .003 in the IVW method, indicating a statistically significant positive correlation between this lipid level and the increased risk of VVs. PC (17:0_20:4) and PC (18:2_20:4) showed statistical significance in all MR methods, suggesting a potential association with a decreased risk of VVs. PC (O-16:0_16:0) and PC (O-17:0_17:1) showed a positive correlation with the risk of VVs in the IVW method, but with higher P -values in the MR-Egger method, indicating that potential pleiotropy might affect the interpretation of these relationships. PE (O-16:1_20:4) and PE (O-16:1_22:5) showed statistical significance in the IVW method on the impact of VVs risk, especially the impact of PE (O-16:1_20:4) was more significant, PI (18:0_18:2) and PI (18:1_18:2) both showed statistical significance related to the risk of VVs, particularly the OR value and P -value of PI (18:1_18:2) indicated a stronger positive correlation. The MR results of these 12 lipids, including their ORs, 95% CIs, and P -values, are presented in Table ​ Table2. 2 . Forest plots, funnel plots, and scatter plots are shown in Figure S1 to S3, Supplemental Digital Content, http://links.lww.com/MD/N476 . The reliability of the above methods is also demonstrated by the BWMR results, which are shown in Table ​ Table3. 3 . Sensitivity testing further confirms the stability of the above results. The results of the sensitivity analysis are presented in Table ​ Table4 4 and in Figure S4, Supplemental Digital Content, http://links.lww.com/MD/N476 .

Association of the genetically predicted plasma levels of lipids with the risk of varicose veins in the lower extremities using BWMR.

BWMR = Bayesian Weighted Mendelian Randomization, PC = phosphatidylcholine, PE = phosphatidylethanolamine, PI = phosphatidylinositol.

Pleiotropy and heterogeneity tests of selected SNPs.

No statistical relationship was found between SM and the risk of VVs. The MR results for the 11 types of SM with VVs are shown in Table ​ Table5 5 .

Associations between the genetically predicted plasma levels of SM and the risk of VVs.

IVW = inverse-variance weighted, SM = sphingomyelin.

Overall, these results support a potential causal relationship between specific types of lipid levels and the risk of VVs.

4. Discussion

This study’s findings reveal potential aspects of the pathogenesis of VVs, emphasizing the role of specific lipid molecules. The results suggest a potential causal relationship between certain types of lipid levels and the risk of VVs. Some lipids, such as PC (15:0_18:1) and PE (O-16:1_20:4), showed significant positive or negative correlations with the risk of VVs. However, for other lipids, this association might be weaker or not significant. These findings provide important clues for future studies on biological mechanisms and potential preventive or therapeutic strategies.

The current understanding of the relationship between plasma lipids and the risk of VVs requires further research. Our study extends existing knowledge about VVs, highlighting the potential causal relationship between specific plasma lipid components and the development of VVs. Previous research by H. Tanaka et al using imaging mass spectrometry revealed a unique lipid distribution in primary varicose veins. They found increased concentrations of PC, Lyso-phosphatidylcholine (LPC), and SM around the veins in patients with VVs[. [ 16 ] LPC is associated with inflammation, being a chemotactic factor for macrophages and lymphocytes, known to induce the expression of Vascular Cell Adhesion Molecule-1 (VCAM-1) and Intercellular Adhesion Molecule-1 (ICAM-1) in endothelial cells. Due to its detergent-like properties, high concentrations of LPC can lyse cells. The specific localization of LPC on the valves of VV tissues suggests that the valves could be damaged due to LPC-induced inflammation or its detergent-like properties. [ 22 ] SM is a precursor of sphingolipid mediators. Over 36 studies have shown that sphingolipid mediators, including ceramides and ceramide 1-phosphate, play an indispensable role in the inflammatory process. The accumulation of PC (1-acyl 36:4) and SM (d18:1/16:0) around the valvular areas may severely exacerbate tissue inflammation around varicose vein valves. Valve dysfunction leads to venous reflux, thereby leading to the development of varicose veins. These lipids are related to inflammation and cellular pathways. The aggregation of PC can lead to the aggregation of phospholipase and its decomposition into LPC and free fatty acids. Furthermore, a study by Anwar et al using analytical techniques provided a comprehensive metabolic profile of the disease. In their study, compared with the non-varicose vein group, the lipid extracts from varicose vein group had higher concentrations of PC, PE, and SM. Pathway analysis indicates that PC and SM are associated with inflammation, while inositol is related to cell proliferation. [ 6 ] SM, PC, phosphocholine, ceramide, and diacylglycerol influence cell survival, with the first 3 promoting growth and survival, and the latter 2 inducing cell death and quiescence. [ 23 , 24 ] This suggests that the local chemical environment and physical stress cells experience following chronic blood stasis could trigger changes in metabolic balance and disrupt lipid homeostasis, leading to the production of certain pro-survival molecules and their downstream effects.

Despite the advantages of well-designed, large-scale prospective studies, they are still susceptible to the effects of residual confounding and reverse causation. Therefore, our study employed MR analysis to explore the relationship between plasma lipid levels and the risk of VVs. In MR analysis, plasma lipid levels are determined genetically; thus, they are not influenced by confounding factors. Utilizing large-scale GWAS data for a two-sample MR analysis provides a more robust method of establishing causality compared to traditional observational studies. The association between 12 types of lipids and the risk of VVs reached statistical significance across various MR analysis methods, enhancing the robustness of these findings. Particularly, when both the IVW and the weighted median methods showed similar significant results, it provided reliable evidence to support the study. For some lipid types, a higher P -value in MR-Egger might indicate potential pleiotropy bias. Careful consideration should be given to potential biases and other confounding factors when interpreting these relationships. Overall, these results support a potential causal relationship between specific types of lipid levels and the risk of VVs. Moreover, the introduction of BWMR addressed potential biases more effectively than traditional MR methods, enhancing the reliability of our findings. The detection of pleiotropy and heterogeneity also supports the robustness and reliability of our results.

Previous research has highlighted the potential role of lipid molecules in the development of VVs, particularly in relation to inflammation and cellular pathways. We have further expanded the research in this area, proposing not only the potential causal relationship between PC and PE types of lipids and VVs but also identifying an association with specific types of PI. Our study provides causal evidence for the role of these lipids in the development of VVs through genetic methods. Although our research did not find a potential causal relationship between SM and VVs, this does not negate the role of SM in the inflammatory process, as was also shown in the studies by Tanaka et al. The differences may stem from the design and analytical methods of our study, highlighting the need for further research to fully understand the complex roles of different lipid molecules in the development of VVs.

This study has some limitations. First, the accuracy of MR analysis highly depends on the validity of the chosen instrumental variables (SNPs). These SNPs must be strongly associated with the exposure and affect the outcome variable only through the exposure. If there are other pathways affecting the outcome, such as horizontal pleiotropy, it might lead to bias. Second, genetic heterogeneity between populations could affect the generalizability of the relationship between SNPs and the exposure, and the GWAS data used in this study primarily come from a specific population (European descent), so the results may not be directly generalizable to people of other backgrounds. Third, MR analysis often assumes a linear relationship between exposure and outcome. If the actual relationship is nonlinear, this simplification could lead to incorrect conclusions. Finally, the quality and accuracy of MR analysis also depend on the quality and availability of the underlying GWAS studies. Any errors or deficiencies in the data could affect the outcomes of the MR analysis. Even if MR analysis indicates a causal relationship, identifying the specific biological mechanisms remains a challenge. Additionally, MR analysis cannot provide information on the size of intervention effects. [ 17 ] Overall, while this study provides valuable insights, these limitations should be considered when interpreting the results and generalizing the findings. Future research may need to be conducted across multiple populations and combined with experimental studies to further validate causal relationships and clarify biological mechanisms.

In conclusion, this study not only advances our understanding of the pathophysiology of VVs but also highlights the potential of lipid molecules as biomarkers and therapeutic targets in vascular diseases. The potential causal links pave the way for further investigation into lipid metabolism’s role in VVs, offering insights for more effective management of this condition.

Author contributions

Conceptualization: Haibin Yu.

Data curation: Kailin Shen, Haibin Yu.

Methodology: Kailin Shen, Haibin Yu.

Resources: Kailin Shen.

Software: Kailin Shen.

Supervision: Fangtao Zhu, Cunwei Cheng, Haibin Yu.

Validation: Kailin Shen.

Visualization: Kailin Shen.

Writing – original draft: Kailin Shen, Fangtao Zhu, Cunwei Cheng.

Writing – review & editing: Kailin Shen, Fangtao Zhu, Cunwei Cheng, Haibin Yu.

Supplementary Material

Abbreviations:.

Scientific and Technological Funding sources: Project of the Education Department of Henan Province.

The authors have no conflicts of interest to disclose.

All data generated or analyzed during this study are included in this published article [and its supplementary information files].

Supplemental Digital Content is available for this article.

How to cite this article: Shen K, Zhu F, Cheng C, Yu H. Exploring the causal relationship between plasma lipids and varicose veins of lower extremity: A comprehensive two-sample Mendelian randomization study. Medicine 2024;103:36(e39514).